基于化学指纹图谱的茶树新品系丽早香识别研究

Identification and characterization of new tea line Lizaoxiang based on chemical fingerprint

化学指纹图谱技术应用于茶树研究可用于其身份、产地的判别。茶树品种间差异鉴别是申请农作物新品种审定的重要内容,试验利用HPLC检测分析丽早香、龙井43、迎霜和当地群体的化学组成及比例,构建化学指纹图谱,从而区分丽早香品系。通过HPLC测定样品色谱数据,选择90%以上样品中存在的峰为样本集的共有峰,建立数字化多元化学指纹图谱。丽早香在图谱相似性上与当地群体最高;在儿茶素、咖啡碱、没食子酸等茶叶主要化学组成比例上,与对照不同,可以与龙井43、迎霜、当地群体辨别区分,为茶树品种间差异鉴别和申请植物新品种保护提供借鉴。

Chemical fingerprint technology has widely been implicated for discriminating the identity and origin of tea plant. Difference identification between tea plant cultivars is important for applying for new variety certification. In the current study,we analyzed the chemical composition and proportion of bioactive compounds using HPLC and built a chemical fingerprint to distinguish Lizaoxiang from Longjing 43,Yingshuang and Jiukeng. On the basis of the obtained HPLC chromatographic data,we selected common peak data of more than 90% samples to build chemical fingerprint chromatograph for further analysis. Chromatography revealed that Lizaoxiang was closest to Jiukeng; while the proportion of catechins,gallic acid and caffeine was different from that of Longjing 43,Yingshuang and Jiukeng.These observations help to distinguish Lizaoxiang from Longjing 43,Yingshuang and Jiukeng,and thus provid reference to characterize the difference between the varieties of tea plant for new variety protection.