摘要
[目的]为了寻找对核桃细菌性疫病及其他细菌有潜在防治作用的天然植物产物。[方法]用滤纸片扩散法,对15种植物的3种溶剂浸提物的抑菌作用进行了室内平板筛选。[结果]除了广玉兰和香蒲之外,其他13种植物叶片浸提物对供试的5个菌株都表现出一定程度的抑菌作用。其中七叶树叶水浸提物对枯草芽孢杆菌XHE8的抑制作用最强,抑菌圈直径达(31.3±3.9)mm,与对照直径之比达5.0以上。接骨草对5个菌株中的4个都表现出明显的抑制作用,麦冬和吉祥草对其中的3个菌株表现出明显抑制作用,它们与对照直径之比都大于1.5。七叶树叶浸提物对细菌有十分显著的抑制作用,有进一步研究和开发的潜力。[结论]研究结果为进一步筛选有效成分及抑菌机理研究奠定了基础。
[Objective] This study was conducted to develop potential natural plant products for controlling walnut blight pathogen and other bacteria. [Method] Inhibitory effects of extracts obtained from 15 plants with 3 solvents on bacteria were investi- gated by disk diffusion method. [Results] Except the extracts from Magnolia grandi- flora and Typha orientalis, extracts of 13 plant leaves presented inhibitory effects on 5 bacteria strains to certain degrees. Among them, the effect of water extract of Aesculu schinensis on Bacillus sp. XHE8 was the strongest, with inhibition zone di- ameter reaching (31.3+3.9) mm and the ratio to control above 5.0. Four of the 5 tested strains were sensitive to the extracts of Sambucus chinensis, and 3 of them were inhibited by Ophiopegon japonicas extracts and Reineckia camea extracts, with ratios of treatment to control large than 1.5 in all. Leaf extract of A. chinensis had significant anti-bacteria ability, and could be used as a potential plant source for bactericide. [Conclusion] The results laid a foundation for exploring active com- pounds and elucidating the mechanism in it.
基金
Supported by National Natural Science Foundation(31200488,31370692)
Surface Project of Natural Science Foundation of Hubei Province(2014CFB573)~~
关键词
植物浸提物
七叶树
接骨草
吉祥草
核桃细菌性疫病
抑制作用
Plant extract
Aesculus chinensis
Sambucus chinensis
Reineckia carnea
Walnut bacterial blight
Inhibition