CD64^+中性粒细胞在烧伤并发感染患者体内变化趋势检测

Detection of Trendency of the CD64^+ Neutrophils in the Patients with Infection Complicated by Burns

本研究旨在探讨CD64^+中性粒细胞在烧伤并发感染患者体内的变化趋势。在2015年3月至2016年3月期间在广西医科大学附属第一临床医学院烧伤科就诊的患者共100例,男性73例,女性27例;其中观察组(并发感染)50例,对照组(未发生感染)患者50例。对两组患者进行血常规检测、烧伤面积统计,流式细胞仪检测两组患者体内CD64^+中性粒细胞在外周血中的比例,并通过荧光显微镜观察两组患者中性粒细胞CD64表达强度的差异性。我们发现观察组患者外周血CD64^+中性粒细胞比例平均高达(41.5±7.6)%,明显高于对照组的(7.2±5.1)%,差异有统计学意义(p<0.05);荧光显微镜下观察结果显示,观察组患者中性粒细胞表面CD64分子表达强度明显高于对照组。因此我们得出结论,烧伤并发感染患者体内CD64^+中性粒细胞比例明显升高,且表达强度明显高于未感染患者,可以用于日后患者感染的诊断及预测。

The aim of this study was to investigate the trendency of the CD64 ＋ neutrophils in the patients with infection complicated by bums. A total of one hundred patients （73 male and 27 female） admitted to our hospital arranged from March 2015 to March 2016 were enrolled in this study. The 100 cases were divided into two groups, 50 cases in observer group and the others in control group, based on whether complicated infection or not. The blood samples and the burn area were detected, and the percentage of CD64＋ neutrophils in the peripheral blood of the two groups were tested by the flow cytometry, and observed the difference of CD64 expression intensity on the surface of the neutrophils bewteen the two groups. We found that in the result the percentage of CD64＋ neutrophils in the observer group reached （41.5±7.6）%, significantly higher than that in the control group with a percentage of （7.2±5. 1）%. Fluorescence microscopy showed that the expression intensity of CD64 on the surface ofneutrophils in the observer group was significantly higher than that in the control group. In conclusion, the percentage of CD64＋neutrophils was significantly increased in the patients with infection complicated by bums, and the expression intensity was significantly stronger than those without infection. So the CD64＋ neutrophils could be used for the prediction and diagnosis of infection.