CDH13基因两个单核苷酸多肽性位点与非小细胞肺癌发生发展的关系

The Relations of the Two Single Nucleotide Polymorphisms Gene Loci of the CDH13 and Non-small Cell Lung Cancer

探讨CDH13基因两个单核苷酸多肽性（SNP）位点（rs11646213和rs7195409）与非小细胞肺癌（NSCLC）发生发展的关系。选取2015年6月～2016年6月我院收治的NSCLC患者100例作为观察组，选取同期健康体检人群100例作为对照组。采用实时荧光定量PCR法检测两组患者的CDH13基因启动子区域两个SNP位点（rs11646213，rs7195409）；分析两个SNP位点在两组中的分布特征。观察组SNP位点rs11646213等位基因频率和基因型频率与对照组比较差异具有统计学意义（p〈0．05），观察组A等位基因频率明显低于对照组（76．2％vs90．4％），差异具有统计学意义（p〈0．05）；SNP位点rs7195409等位基因频率和基因型频率在临床Ⅰ＋Ⅱ期和Ⅲ＋Ⅳ期患者间比较差异具有统计学意义（p〈0．05），Ⅰ＋Ⅱ期患者G等位基因频率明显高于Ⅲ＋Ⅳ期患者（25．8％vs12．4％），差异具有统计学意义（p〈0．05）。CDH13基因SNP位点rs11646213的A等位基因缺失可能与NSCLC发生启动有关，SNP位点rs7195409的G等位基因缺失可能与NSCLC的病情发展有关。

The objective of the study was to explore the relations between the two single nucleotide polymorphisms （SNP） loci （rs11646213 and rs7195409） of the CDH13 gene and the occurrence and development of non-small cell lung cancer （NSCLC）. We selected 100 cases of patients with NSCLC from June 2015-June 2016 as the observa- tion group, selected the 100 cases of health check-up crowd at the same period as control group. We detected the two SNP loci （rs11646213, rs7195409） in CDH13 gene promoter regions of the two groups of patients by the real- time fluorescent quantitative PCR method and analysised the distribution characteristics of the two SNP locus in two groups. The allele frequency and genotype frequency of the SNP loci rs11646213 in the observation group had statistically significant differences compared with controls group （p〈0.05）, A allele frequency of the observation group was obviously lower than the control group （76.2% vs 90.4%）, the difference had statistically significant （p〈 0.05）; The allele frequency and genotype frequency of the SNP loci rs7195409 in clinical stage I ＋ II had statistically significant differences compared with the clinical stage III ＋IV patients （p〈0.05）, G allele frequency of the clinical stage I ＋ II patients was significantly higher than clinical stage II ＋IV patients （25.8% vs 12.4%）, had statistically significant difference （p 〈0.05）. The conclusion of the study was that the alleles deficiency of the SNP loci rs 11646213 of CDH13 gene may be associated with the startup of NSCLC; Galleles deficiency of the SNP loci rs7195409 may be associated with the progression of the NSCLC.