植物液泡蛋白质组研究

Research Progress on Plant Vacuole Proteomics

植物液泡是植物生长、发育及逆境防御必要细胞器。细胞中有不同类型的液泡,执行不同的功能,如蛋白质降解、更新、解毒、代谢物储存和离子稳态等。随着质谱技术的快速发展,基于质谱的蛋白质组学分析将有助于鉴定液泡蛋白质组分及了解液泡膜上的一些特异载体和通道转运过程。本综述将从液泡功能,液泡蛋白质及液泡膜蛋白的提取、纯化,植物液泡蛋白质组及膜蛋白质组的研究,液泡磷酸化蛋白质组研究等方面进行阐述。

Plant vacuoles are necessary for plant growth, development and defense. There are different types of vacuoles in cells, which perform different functions such as protein degradation and regenation, detoxification, storage of metabolites and control of ionic homeostasis. Mass spectrometry-based proteomic analysis will help to identify and understand vacuole/tonoplast protein components and transport processes of specific carriers and channels. The article reviewed the vacuole functions, extraction and purification of vacuole/tonoplast proteins, research progress ofvacuole/tonoplast proteomics and vacuole/tonoplast phosphoproteome.