兔抗猪繁殖与呼吸综合征病毒血清在Marc-145细胞上对病毒复制影响

Effect of antiserum of PRRSV on the replication of PRRSV in Marc-145 cells

制备兔抗猪繁殖与呼吸综合征病毒抗血清,探索其对病毒复制影响,通过大量培养猪繁殖与呼吸综合征病毒XH-GD株病毒,高速离心后蔗糖梯度纯化浓缩并免疫新西兰大白兔,4免后采集抗血清,ELISA法测定效价。同时将抗血清与病毒共同孵育接种Marc-145细胞,荧光定量PCR和TCID50测定病毒复制情况。成功制备兔抗猪繁殖与呼吸综合征病毒抗血清,效价达1640,抗血清可以在m RNA水平和TCID50上降低病毒滴度。结果表明,兔抗猪繁殖与呼吸综合征病毒抗血清可以在Marc-145细胞上抑制猪繁殖与呼吸综合征病毒复制。

In order to verify whether hyper-immune serum of PRRSV has an influence on the replication of PRRSV, hyper-immune serum was prepared, PRRSV XH-GD strain were cultured, then centrifuged with high speed, the purified viruses were used to immunize the New Zealand rabbits to raise antibody. New Zealand rabbits were immunized intraperitoneally （i.p.） plus Freund＇s complete adjuvant. Antiserum was collected from the rabbits that had been placed under terminal halothane anesthesia. Antiserum titer was 1 ： 640, the antiserum was incubated with virus, then qPCR and TCID50 used to evulate the titer of virus. The results showed that antiserum could inhibit the replication of PRRSV at the mRNA lever, so hyper-immune serum of PRRSV was able to inhibit the replication of PRRSV in Marc-145 cells.