雷帕霉素通过诱导细胞自噬调控调节性T细胞的分化及功能

Rapamycin Regulateing Treg Cells Differentiation and Function Via Autophagy

目的探究雷帕霉素Treg细胞的调控作用是否与细胞自噬有关。方法取小鼠脾脏,经研磨后用细胞分选试剂盒分选细胞。细胞经抑制剂或干扰分子处理改变细胞自噬水平。通过流式细胞术检测Treg细胞的生成比例。结果抑制自噬Treg细胞生成比例显著下降。结论细胞自噬参与调控Treg细胞功能。

Objective To explore whether the effect of Rapamycin on the Treg cells is related to autophagy. Methods the mice spleen was gently minced and the cells were sorted by the cell sorting kit. Inhibitors or siRNA were used to inhibit or induce the cellular autophagy. The proportion of Treg cells was detected by flow cytometry. Results Inhibited autophagy down- regulates the proportion of Treg cells. Conclusion Autophagy participates in the regulation of Treg cells function.