糖尿病神经痛大鼠脊髓背角和背根神经节Caveoline-1蛋白表达量的变化

Caveoline-I protein expression in spinal cord dorsal horn and dorsal root ganglia in diabetic pe- ripheral neuropathy pain model rats

目的观察在糖尿痛周围神经病变大鼠疼痛模型中不同作用时间脊髓背角和背根神经节Caveolin-1表达量的变化。方法健康成年雄性sD大鼠50只，随机选取20只大鼠为正常对照组，腹腔注射50mg／kg氯化钠注射液；余30只腹腔注射50mg／kg链脲佐菌素（STZ）制备糖尿病大鼠模型，注射STZ1周后，测大鼠尾静脉随机血糖，〉16．7mmol／L者为糖尿病造模成功大鼠，2周后，测定糖尿病大鼠的50％机械缩足阈值（PWT），将50％机械缩足阈值〈4g的大鼠作为糖尿病神经痛造模成功大鼠。于注射STZ后4、6、8周每组选取5只大鼠，处死取第5腰椎脊髓和背根神经节，用western-blot的方法检测脊髓背角和背根神经节Caveolin-1的表达量。结果STZ造模后4、6、8周糖尿病神经痛大鼠脊髓背角Caveoline．1蛋白表达量与对照组相比分别升高了（144．40±6．04）％、（105．50±5．12）％、（177．80±5．23）％，差异有统计学意义（P〈0．05）。背根神经节处Caveoline-1蛋白表达量与对照组相比分别升高了（128．60±6．51）％、（160．00±6．72）％、（120．10±6．18）％，差异有统计学意义（P〈0．05）。结论糖尿病神经损伤引起的慢性痛可诱导脊髓背角和背根神经节Caveolin-1的表达量的增加。

Objective To observe the Caveoline-1 protein expression in spinal cord dorsal horn and dorsal root ganglia in diabetic peripheral neuropathy pain model rats at each observing point after STZ injection. Methods Fifty male SD rats were randomly devieded into two groups, 20 rats in control group were administrated with normal saline, and the other 30 rats were administrated with STZ（50 mg/kg） by introperitoneal injection. After one week of administration, the random blood glucose level from caudal vein was detected, and the rats whose random blood glucose were above 16. 7 mmol/L were selected for fur- ther study, the index of paw withdrawal threshold （PWT） after two weeks of administration were tested, then the rat with PWT less than 4 g were selected. At each observing point, five rats were selected at the fourth, the sixth and eighth week after STZ injection to detect the Caveoline-1 protein expression in spinal cord dorsal horn and dorsal root ganglia with western-blot method. Results The Caveoline-1 protein ex- pression in diabetic peripheral neuropathy pain model rats increased by （ 144. 40 ±6.04） %, （ 105.50± 5.12） % , （177.80 ± 5.23 ）% , compared with the control group at four, six week and eight weeks after STZ injection in spinal cord dorsal horn, the differences were significant （P 〈 0. 05 ）. The Caveoline-1 protein expression in diabetic peripheral neuropathy pain model rats increased by （128.60± 6. 51 ）%, （ 160. 00 ± 6. 72） %, （ 120. 10± 6. 18 ） %, compared with the control group at each observing point after STZ injection in dorsal root ganglia, the differences were significant （P 〈 0. 05 ）. Conclusions The chronic pain caused by diabetes nerve damage can induce the increased amount of the expression of Cave- olin-1 on spinal cord dorsal horn and dorsal root ganglia.