血管生成拟态和上皮-间质转化相关标志物在鼻咽癌中的表达及与淋巴结转移的关系

Expression of vasculogenic mimicry and epithelialmesenchymal transition-related markers in nasopharyngeal carcinoma and its relationship with lymphnode metastasis

目的研究鼻咽癌中血管生成拟态（vasculogenic mimicry,VM）和上皮-间质转化（epithelialmesenchymal transition,EMT）相关标记物的表达及与淋巴结转移的关系。方法免疫组化检测82例鼻咽癌和20例鼻咽慢性炎组织E-cadherin、Twist和Vimenitn表达,CD31联合PAS染色检测VM。结果 VM在鼻咽癌组织的阳性率为28.05%,与淋巴结转移具有正相关关系（χ-2=6.927,P=0.008）。27例出现E-cadheirn表达减弱甚至缺失,23例和35例出现Vimentin和Twist阳性表达,E-cadherin表达与淋巴结转移具有密切负相关关系（χ-2=9.694,P=0.002）,Vimentin和Twist表达均与淋巴结转移具有密切正相关关系（χ-2=6.411,P=0.011;χ-2=14.576,P=0.000）,VM和EMT标志物具有密切相关性（P均〈0.000）。结论鼻咽癌中存在VM和EMT,存在VM和发生EMT的鼻咽癌易发生淋巴结转移,VM和EMT具有密切相关性,两者对鼻咽癌转移具有一定的预测价值。

OBJECTIVE To study the existence of vasculogenic mimicry（VM） and the expression of epithelialmesenchymal transition（EMT）-related markers,and their relation with the lymph node metastasis of nasopharyngeal carcinoma（NPC）.METHODS The expression of E-cadherin,Twist and Vimentin protein were detected by immunohistochemistry in 82 samples of NPC and 20 samples of inflammatory nasopharyngeal mucosa.The presence of VM was observed by CD31 combined with PAS stain.The clinical significance of EMT and VM were then analyzed using statistical method.RESULTS The positive rate of VM was 28.05%（23/82） in NPC.The bivariate analysis showed VM is positively correlated with lymphatic metastasis in NPC（χ-2=6.927,P=0.008）.E-cadherin expression was down-regulated in 27 NPC cases,and the expression of Vimentin and Twist were upregulated in 23 and 35 cases respectively.The bivariate analysis showed EMT-related markers were closely correlated with lymphatic metastasis in NPC（χ-2=6.411,P=0.011;χ-2=14.576,P=0.000）.There was a positive correlation between the expression of VM and EMT-related markers（all P〈0.000）.CONCLUSION VM and EMT exists in NPC and correlated with the lymphatic metastasis.VM is closely correlated with EMT,they had predictive value for the prognosis of NPC.