不同激素和外植体对柱花草愈伤组织诱导及体细胞胚的影响

Effect of Different Hormone and Explants on Callus Induction and Somatic Embryo Differentiation of Stylosanthes spp.

以MS为基本培养基,以柱花草无菌苗的幼根、下胚轴、子叶、茎和真叶为材料,采用正交设计方法,研究不同外植体和激素对柱花草愈伤组织诱导和体细胞胚的影响。结果表明:外植体类型及2,4-D、KT、NAA、6-BA浓度对愈伤组织诱导率的影响均达极显著水平(P<0.01),其中2,4-D浓度是最主要的影响因素,其次为外植体类型。下胚轴和真叶为柱花草愈伤组织诱导的理想外植体,愈伤诱导培养基的最优配方为MS+1.0mg/L 2,4-D+0.5mg/L KT+0.01mg/L NAA+0.1mg/L 6-BA。NAA、6-BA浓度对柱花草体细胞胚产生率的影响达极显著水平(P<0.01),NAA浓度是主要影响因素,体细胞胚产生的最佳培养基配方为MS+1.0mg/L NAA+1.0mg/L 6-BA+2.0g/L CH。

The root,hypocotyls,cotyledon,stem and leaf of Stylosanthes spp.were taken as the explants for researching the effects of different hormone compositions on callus and somatic embryos induction.The results indicated that the callus induction were effected significantly by explants,different exogenous hormone and concentration,in which,the main effecting factor of the callus induction was 2,4-D,the second was explants types.Hypocotyls and leaf was the ideal explants material.The optimum medium for callus induction was MS＋1.0mg/L 2,4-D＋0.5mg/L KT＋0.01mg/L NAA＋0.1mg/L 6-BA.The main effecting factor of somatic embryos induction was NAA,the optimum medium for somatic embryos differentiation was MS＋1.0mg/L NAA ＋1.0mg/L 6-BA＋2.0g/L CH.