不同液相色谱仪测定辣椒油中维生素E的含量分析

Comparison of the content of vitamin E in pepper oil by different liquid chromatograph

目的建立不同液相色谱仪测定辣椒油中维生素E的含量测定方法。方法在相同试验条件下,采用不同液相色谱仪测定辣椒油中维生素E的含量。以维生素E为标准物质,C18柱（4.6 mm×250 mm,5μm）为分离柱,甲醇为流动相洗脱,紫外检测,流速1.0 ml/min。结果维生素E线性范围10~80μg/ml,3台仪器测得维生素E的相关系数分别为0.9978、0.9990、0.9998,加标回收率和相对标准偏差（RSD）分别为97.8%、1.8%,98.2%、0.92%,99.4%、0.53%。结论超高效液相色谱法试剂简单,操作简便,结果重现性好,准确快速,回收率高,适用于辣椒油中维生素E含量的测定。

Objective Establish different liquid chromatograph the content determination method for determination of vitamin E in chili oil.Methods Under the same experimental conditions,the analytical method of the content of vitamin E in chili oil was determined by different instruments.Vitamin E as a standard substance,C18 column（250 mm×4.6 mm,5 μm）as Separation column,Methanol as mobile phase elution,UV detection,Flow rate of 1.0 ml/min.Results the linear range of vitamin E was between 10-80 μg/ml,The correlation coefficients of vitamin E were measured by three instruments were 0.9978,0.9990,0.9998,the recovery rate and relative standard deviation were 97.8%,1.8%, 98.2%,0.92%,99.4%,0.53%.Conclusion Ultra performance liquid chromatography reagent is simple and easy to operate.The results are reproducible,accurate,rapid and high recovery rate.It is suitable for the determination of vitamin E in chili oil.