摘要
目的 研究microR NA-590-3p（miR-590-3p）对人胃癌SGC-7901细胞增殖、迁移和侵袭的影响及可能机制。方法 从石蜡包埋的11例胃癌组织和11例正常组织中提取总RNA,应用Real-time PCR方法检测miR-590-3p的表达水平;培养胃癌细胞SGC-7901,应用Lipofectamine LTX试剂将化学合成的miR-590-3p antagomir和miR-590-3p agomir转染SGC-7901细胞,验证转染效率后应用MTT发检测细胞增殖能力的变化;应用Transwell小室法检测SGC-7901细胞迁移和侵袭能力的变化;应用Western Blot方法检测上皮钙粘素（E-cadherin）的蛋白表达水平。结果 与正常组织相比,miR-590-3p在胃癌组织中的表达水平显著增高。与对照组相比,miR-590-3p表达沉默能够显著抑制人胃癌SGC-7901细胞的增殖、迁移和侵袭能力,显著增加E-cadherin的蛋白表达水平。miR-590-3p过表达的作用与之相反。结论 MiR-590-3p沉默抑制胃癌SGC-7901细胞的增殖,迁移和侵袭可能与上调E-cadherin蛋白表达相关。
Objective To study the effects and potential mechanism of microRNA-590-3p（miR-590-3p） on the proliferation, migration and invasion of gastric cancer cells SGC-7901. Methods Total RNA was extracted from 11 cases of gastric cancer tissues and normal tissues, respectively. The mRNA expression level of miR-590-3p was detected by real- time PCR. After gastric cancer cells SGC-7901 were cultured, Lipofectamine LTX was applied to transfecting gastric cancer cells SGC-7901 with miR-590-3p antagomir and miR-590-3p agomir. After the transfection efficiency was proved by real- time PCR, MTT was applied to detect the viability of gastric cancer cells SGC-7901. Transwell chamber assay was used to measure the migration and invasion of gastric cancer cells SGC-7901. The expression of E-cadherin protein was detected by Western blot. Results The expression level of miR-590-3p was significantly higher in gastric cancer tissues than in normal tissues. MiR-590-3p silence significantly inhibited the proliferation, migration and invasion ability of gastric cancer cells SGC-7901 and obviously increased the expression level of E-cadherin protein, but with opposite role for miR-590-3p overexpression. Conclusion The inhibition effect of MiR-590-3p silencing on the proliferation, migration and invasion of gastric cancer cells SGC-7901 might be related to the upregulation of E-cadherin.
出处
《解剖科学进展》
2016年第6期591-595,共5页
Progress of Anatomical Sciences
基金
国家自然科学基金(81402573)
