Ⅱ型囊泡谷氨酸转运蛋白在SD大鼠胰腺的表达

Expression of vesicular glutamate transporter type 2 in the pancreas of SD rat

目的研究Ⅱ型囊泡谷氨酸转运蛋白(vesicular glutamate transporter type 2,VGLUT2)在SD大鼠胰腺组织中的表达。方法采用双重荧光免疫组织化学的方法,观察VGLUT2在大鼠胰腺组织中的分布。结果 VGLUT2免疫反应阳性细胞分布在SD大鼠胰腺内分泌腺A细胞、B细胞和部分PP细胞,阳性物质分布于细胞质,D细胞及部分PP细胞无VGLUT2免疫活性。结论 VGLUT2在大鼠胰腺的表达提示VGLUT2可能在胰腺起着非常重要的作用。

Objective To investigate the distribution of vesicular glutamate transporter type 2 （VGLUT2） in the pancreas of normal SD rat. Methods Immunofluorescence histochemical double-labeling technique was used to observe the localization of VGLUT2 in the pancreas of normal SD rats under a confocal laser-scanning microscope. Results VGLUT2 positive immunoreactivity was localized in the cytoplasm of glucagon-secreting cells（A cells） and insulin-secreting cells（B cells） and part pancreatic polypeptide（PP）-secreting cells（PP cells）, but not in D cells. Conclusion VGLUT2 might play a critical role in the pancreas.