不同时期局部移植人脐带间充质干细胞修复脊髓损伤的组织学观察

Local transplantation of human umbilical cord mesenchymal stem cells at different time after spinal cord injury in rats: a histological observation

背景:脊髓损伤能造成各种运动、感觉功能障碍,肌张力异常及病理反射等,临床上已通过人脐带间充质干细胞移植对脊髓损伤进行治疗。目的:探讨大鼠脊髓损伤后不同时期局部移植人脐带间充质干细胞修复脊髓损伤的效果。方法:选取SPF级雄性成年SD大鼠100只,随机分为5组:空白对照组、单纯脊髓损伤组及3,7,21 d后移植组,各20只。后4组利用Allen’s打击器制作大鼠T10脊髓损伤模型;3,7,21 d后移植组大鼠分别于脊髓损伤后3,7和21 d在损伤部位周围移植人脐带间充质干细胞。结果与结论:与单纯脊髓损伤组相比,3,7,21 d后移植组大鼠造模后49 d运动功能评分增加,血清白细胞介素2水平减少,血清白细胞介素10水平增加,脊髓病理损伤明显改善,且7 d后移植组效果最为明显。说明人脐带间充质干细胞移植修复大鼠脊髓损伤,改善大鼠运动功能,且在大鼠脊髓损伤7 d后移植效果最佳。

BACKGROUND：Spinal cord injury （SCI） is a disease causing a variety of motor and sensory dysfunctions, abnormal muscle tone and pathological reflex. Clinical y, human umbilical cord mesenchymal stem cel transplantation has become an employed therapy for SCI. OBJECTIVE：To investigate the effect of local transplantation of human umbilical cord mesenchymal stem cel s in different time after spinal cord injury in rats. METHODS：100 SPF male adult Sprague-Dawley rats were randomly divided into five groups：blank control group, SCI group, post-SCI 3-, 7-, 21-day transplantation groups （n=20 per group）. Animal models of T10 SCI were made by Al en’s method in the latter four groups, and rats in the three transplantation groups were given HUCMSCs transplantation at 3, 7, 21 days after SCI, respectively. RESULTS AND CONCLUSION：Compared with the SCI group, improved motor function scores, decreased interleukin-2 level, and increased serum interleukin-10 level were observed in the three transplantation groups at 49 days after modeling, indicating SCI was improved significantly in the three transplantation groups, especial y in the post-SCI 7-day transplantation group. These findings suggest that human umbilical cord mesenchymal stem cel transplantation for SCI repair improves the movement function of rats, and cel transplantation at 7 days after modeling has achieved best outcomes.