复方地芬诺酯建立大鼠便秘模型及对肠道菌群的影响

Effect of constipation induced by diphenoxylate on intestinal flora in a rat

背景:既往研究中检测便秘模型的指标多为粪便粒数、肠道张力变化等整体指标,很少涉及肠道菌群的变化。目的:以复方地芬诺酯建立便秘大鼠模型探索便秘对大鼠肠道张力、病理及微生态等方面的影响。方法:20只SPF级Wistar大鼠随机分为空白组和模型组,分别连续20 d灌胃10 mg/kg的复方地芬诺酯和蒸馏水。结果与结论:与空白组相比,给药20 d,模型组大鼠回肠收缩频率降低,24 h排便量减少,盲肠内容物中细菌种群结构存在变化,结肠结构严重破坏。结果表明,便秘大鼠肠道菌群严重紊乱。

BACKGROUND： In the previous studies, the overall index in constipation models, such as fecal output in 24 hours and intestinal tension are usually measured. However, the changes of intestinal flora are rarely involved. OBJECTIVE： To explore the effect of constipation on intestinal tension, pathology and microecology in the rat models of diphenoxylate-induced constipation. METHODS： Twenty SPF Wistar rats were randomly divided into blank control and model groups, followed by given 10 mg/kg diphenoxylate and distilled water by gavage once daily for 20 days, respectively. RESULTS AND CONCLUSION： Twenty days later, compared with the blank control group, the frequency of ileum contraction was decreased, 24-hour stool decreased, the ratio of bacteria group in the cecum changed, and structure of the colon was seriously damaged in the model group. These results suggest that constipation results in the intestinal flora disturbance remarkablely.