石菖蒲对抑郁模型大鼠海马组织mTOR信号通路相关蛋白表达的影响

Effects of Rhizoma Acori Tatarinowii(Shi Chang Pu)on expression of mTOR signaling pathway related protein in hippocampus of depression model rats

目的:探讨石菖蒲对抑郁模型大鼠海马组织mTOR信号通路相关蛋白表达的影响。方法:采用孤养结合慢性不可预见应激方法造成大鼠抑郁,在7、14、21、28d时,检测各组大鼠旷场实验中水平运动、垂直运动得分,并用Western印迹法检测各组大鼠海马组织mTOR信号通路相关蛋白的表达变化。结果:21d、28d时,与对照组比较,模型组垂直运动和水平运动得分减少(P<0.01);与模型组比较,中、西药组垂直运动和水平运动得分增加(P<0.05、0.01)。21d、28d时,与对照组比较,模型组大鼠海马Akt、mTOR、p70S6K和elF-4E蛋白的表达下调(P<0.01);与模型组比较,中、西药组大鼠海马Akt、mTOR、p70S6K和elF-4E蛋白表达增加(P<0.01)。结论:石菖蒲的抗抑郁效应可能通过激活mTOR信号通路相关蛋白的表达而实现。

Objective：To explore the effects of rhizoma acori tatarinowii on mTOR signaling pathway in the hippocampus of depression model rats.Methods：The rat models with depression induced by isolation and chronic unpredictable stress were studied.Models were randomly divided into four groups and the rats of each group were intragastrically perfused with corresponding interventions at the 7th,14 th,21st,and 28 th day.The horizontal movement and vertical movement scores in the open field test were got,and the expressions of mTOR protein and Akt,p70S6 K,elF-4Eprotein in the hippocampus were examined by Western blotting.Results：On the 21 st and 28 th day,the horizontal motion and vertical motion scores in the open field test of the model group was lower than those of the control group（P〈0.01）.The horizontal motion and vertical motion scores in the open field test of Chinese medicine group and western medicine group were higher than those of the model group（P0.05 and P〈0.01）.On the 21 st and 28 th day,mTOR,Akt,p70S6 K or elF-4E protein expressions in the hippocampus of the rats in the model group were lower than those in the control group（P〈0.01）.The expressions of mTOR protein and Akt,p70S6 K,elF-4Eprotein in the hippocampus of the rats in Chinese medicine group and western medicine group were higher than those in the model group（P〈0.01）.Conclusions：The anti-depressant effect of rhizoma acori tatarinowii can be realized by upregulating mTOR,Akt,p70S6 Kor elF-4Eprotein expressions in the hippocampus.