牙髓干细胞修复即刻种植周围骨缺损

Dental pulp stem cells repair the immediate implantation for peri-implant bone defects

目的:建立兔下颌骨前牙区即刻种植种植体周围骨缺损的动物实验模型,并观察牙髓干细胞在种植体周围骨缺损中骨再生能力。方法:将实验兔分为2组,分别拔除兔双侧下颌前牙,并在拔牙窝颊侧建立2 mm×3 mm大小骨缺损区,即刻植入种植体。对照组植入Bio-oss骨粉,实验组植入Bio-oss骨粉与牙髓干细胞(Dental Pulp Stem Cells,DPSCs),通过扫描电镜和HE染色观察评价植入后4周种植体-骨结合状况。结果:扫描电镜观察见实验组种植体周围骨缺损处可见编织骨及骨小梁形成,种植体与牙槽窝间隙基本消失且与龈方间隙减小,周围松质骨密度增高。实验组HE染色切片见种植体周围骨缺损处牙槽骨部分胞质呈空泡状,成骨细胞与破骨细胞分布于骨小梁上,骨小梁致密且排列规则。结论:建立的兔下颌骨前牙区即刻种植的种植体周围骨缺损动物实验模型,可为即刻种植方向的相关研究提供重要参考。

Objective： To establish an animal experimental model of the immediate implantation for peri-implant bone defects of the rabbit mandibular anterior teeth,and observe dental pulp stem cells to repair peri-implant bone defects. Methods： The rabbits that the bilateral mandibular anterior teeth were extracted respectively were divided into 2 groups. Establishing bone defect of 2 mm × 3 mm area on the buccal side of tooth extraction socket,and the implant was implanted immediately,that the control group was implanted with Bio-oss and the experimental group with Bio-oss and dental pulp stem cells（ DPSCs）. The implant-osseointegration condition could be evaluated by scanning electron microscope,HE staining. Results：Through scanning electron microscope,the formation of visible woven bone and trabecular bone on implant osseointegration interface of the experimental group could be observed,and the gap between implant and alveolar fossa basically disappeared and between the implant and gingival side decreased,the density of cancellous bone around the implant increased as well. HE staining showed that the cytoplasmic portion of alveolar bone around the implant was vacuole,and the osteoblasts and osteoclasts were distributed on the trabecular bone,the trabecular bone was compact and regularly arranged. Conclusion： The establishment of an animal experimental model of the immediate implantation for peri-implant bone defects of the rabbit mandibular anterior teeth can provide an important reference for the relevant research about the immediate implantation direction.