P120^(ctn)/E-cad在口腔鳞癌细胞迁移和侵袭中的功能

The function of P120^(ctn)/ E-cad in the metastasis and invasion of oral squamous-cell cancer

目的:分析P120-连环蛋白(P120-catenin,P120~ctn)和E-钙粘蛋白(E-cadherin,E-cad)在口腔鳞癌(oral squamous-cell cancer,OSCC)细胞迁移和侵袭中的功能。方法:采用实时荧光定量PCR、Western blot检测HN4(人舌癌原发灶细胞)和HN12(人舌癌转移淋巴结细胞)中P120~ctn、E-cad的mRNA和蛋白表达,通过Transwell细胞侵袭及细胞迁移试验等方法检测HOK(人口腔黏膜角化上皮细胞)、HN4和HN12细胞的迁移和侵袭能力。结果:HN4细胞中P120~ctn和E-cad的mRNA和蛋白表达水平明显高于HN12,低于HOK细胞,差异均有统计学意义(P<0.05)。HN4细胞的侵袭和转移能力显著高于HOK细胞,低于HN12细胞,差异均有统计学意义(P<0.05)。结论:在OSCC细胞中P120~ctn与E-cad的表达下调,可能抑制了OSCC细胞的侵袭和转移。

Objective： To reveal the function of P120-catenin（ P120ctn） and E-cadherin（ E-cad） in the metastasis and invasion of oral squamous-cell cancer（ OSCC）. Methods： Real-time fluorescent quantitative PCR and Western blot were adopted to test mRNA and protein expressions of P120 ctnand E-cad in HN4（ originating from a primary OSCC of the tongue）,HN12（ derived from a lymph node metastasis in the same patient） cells. Transwell cell invasion and migration assay were used to test the invasion and migration capacity of HOK（ Human oral keratinocytes）,HN4 and HN12 cells. Results： The mRNA and protein expressions of P120 ctnand E-cad in HN4 cells were significantly higher than those in HN12 cells,and lower than those in HOK cells（ P 0. 05）. The metastasis and invasion capacity of HN4 cells was significantly higher than that of HOK cells,and significantly lower than that of HN12 cells（ P 0. 05）. Conclusion： In OSCC cells,P120 ctnand E-cad which present coordinated expression are related with the malignancy degree and metastatic potential of OSCC epithelial cells,and maybe inhibit the metastasis and invasion of OSCC epithelial cells.