人肺腺癌组织S100A6蛋白表达及其临床意义

Clinical significance of S100A6 protein expression in human lung adenocarcinoma

目的 探讨 S100A6 蛋白表达对肺腺癌患者的临床特征及其转移预后的影响。方法 收集手术切除、经病 理 证实 的肺 腺癌 及 配对 癌旁 正常 肺 组织 标 本 各 98 例,通 过 Westernblot 检 测S100A6 蛋白表达。结果 肺腺癌组织 S100A6 蛋白表达量显著高于癌旁正常肺组织 （ t =19.884 , P 〈0.05 ）; S100A6 蛋白表达与肺腺癌患者性别、年龄无相关性 （ P 值均 〉0.05 ）,与患者吸烟指数、肿瘤细胞分化、淋巴结转移、远处转移、临床分期均有显著相关 （ P 值均 〈0.05 ）; Kaplan-Meier 法比较低表达组和高表达组患者的生存期,差异有统计学意义 （ P 〈0.05 ）; COX 比例风险多因素回归分析发现,肿瘤分化、 TNM 分期和 S100A6 蛋白表达是影响肺腺癌患者预后的独立因素。结论 S100A6在肺腺癌组织显著高表达,可以作为与肿瘤发生、发展密切相关的一种标志蛋白。

Objective To explore the clinical and pathological significance of expression of S100A6 protein in lung adenocarcinoma. Methods S100A6 protein expression in 98 cases of lung adenocarcinoma tissues pathologically confirmed and the matched normal lung tissues was detected by Western blot method. Results The expression of S100A6 protein in lung adenocarcinoma tissues was higher than that in the adjacent normal lung tissues （ t = 19. 884, P 〈0.05）. The expression of S100A6 protein in lung adenocarcinoma was not related to the sex and age of patients （all P 〉 0.05）, but was significantly correlated with smoking index, tumor cell differentiation, lymph node metastasis, distant metastasis and clinical stage （all P 〈0.05）. There was significant difference in the survival time between low expression group and high expression group （ P 〈0.05）. COX multivariate proportional hazard regression analysis revealed that tumor differentiation, TNM stage and S100A6 protein expression were some independent prognosis factors of lung adenocarcinoma. Conclusions S100A6 is over-expressed in lung adenocarcinoma tissue and could be a valuable biomarker to predict tumor occurrence and development.