熊本牡蛎单体苗种生产技术研究

Establishment of Single Oyster(Crassostrea sikamea)Seeds

采用肾上腺素诱导法和先固着后脱基法,研究了熊本牡蛎(Crassostrea sikamea)单体苗种生产技术,确定了单体苗种的最佳生产方法。研究表明:采用肾上腺素诱导法生产单体熊本牡蛎,诱导效果与肾上腺浓度、诱导时间、幼虫密度有关。浓度实验显示,1×10^(-4) mol/L是单体熊本牡蛎最佳诱导浓度;诱导时间实验显示,1h是单体熊本牡蛎最佳诱导时间;密度实验显示,1 000ind/mL是单体熊本牡蛎最佳诱导密度。采用先固着后脱基法生产单体熊本牡蛎,结果显示:灰色聚乙烯波纹板生产单体牡蛎,幼虫易附着,剥离方便,单体率显著高于筛绢网、塑料薄膜、网衣和聚丙烯扁条(P<0.05)。经过25天培育,单体稚贝和固着稚贝壳高分别为(1 953±395)和(1 875±660)μm,单体牡蛎游离状态对生长速度无显著影响(P>0.05)。研究结果显示,药物诱导法稚贝中间培育需要特殊设施,不利于生产技术推广;以灰色聚乙烯波纹板作为附着基,利用先固着后脱基法生产单体牡蛎,是一种可行的熊本牡蛎单体苗种生产技术。

Drug treatment in combination with epinephrine and fixation-removal method were used to establishing the production technique of single oyster（Crassostrea sikamea）seeds.The performance of single seeds production with the method of epinephrine treatment was concentration,time and larval density dependent.We found that 0.1mmol/L was the best for single oyster seed production;1hour treatment was the optimum for single oyster seed production;and the best density was 1000inds/mL.Gray corrugated polyethylene（PE）board was advent;it was high in settlement rate and easy to operate.It performed significantly better higher than bolting-cloth net,plastic film,nylon rope and polypropylene packing tape（P〈0.05）.After cultivating for 25 days,the mean shell height of single seeds and attached spats were（1 953±395）and（1 875±660）μm,respectively.No significant effect on growth speed of single seeds was found（P〈0.05）.As the juvenile cultivating of single oyster by drug treatment need special facilities,it not conducive to the promotion of the production technology.The production using the gray corrugated PE board as the attachment base can efficiently produce single oyster seeds.