野鸡尾体外止血活性部位的筛选研究

Screening of the Effective Fraction with Hemostatic Effect in vitro from Onychium Japonicum

目的:确定野鸡尾的体外止血活性部位,为阐明其止血活性物质基础提供实验依据。方法:以云南白药作为阳性药物(5 mg·ml^(-1)),设空白对照(去离子水)组,采用试管法、凝血板法测定野鸡尾乙醇提取物、石油醚萃取物、乙酸乙酯萃取物、正丁醇萃取物及水提取物(5 mg·ml^(-1))对体外血浆复钙时间和凝血时间的影响,并以体外血浆复钙时间和凝血时间为筛选指标对各部位止血效果进行评价。结果:与空白对照组比较,野鸡尾乙酸乙酯萃取物对缩短体外血浆复钙时间和凝血时间的作用效果显著,且优于云南白药(P<0.01);野鸡尾乙醇提取物可在一定程度上缩短体外血浆复钙时间和凝血时间(P<0.01);野鸡尾石油醚萃取物及正丁醇萃取物对缩短体外血浆复钙时间无明显作用(P>0.05),对体外凝血时间有缩短作用(P<0.01);野鸡尾水提取物在缩短体外血浆复钙时间和凝血时间方面效果均不显著(P>0.05)。结论:野鸡尾乙酸乙酯萃取物具有较好的止血作用,为主要体外止血活性部位。

Objective： To study the in vitro hemostatic effects of different extracts of Onychium japonicum. Methods： In the experiment, there was a blank control group （deionized water）, positive drug Yunnan Baiyao group （5 mg· ml^-1 ） and ethanol extract, petroleum extract, acetic ether extract, n-butanol extract and water decoction of O. japonicun groups （5 mg· ml^-1 ）. A test tube method and a plate method for blood coagulation were adopted to determine the plasma recalcification time and the coagulation time in vitro. Results： Compared with blank group, acetic ether extract of O. Japonicun could significantly shortened the plasma recalcification time and coagulation time in vitro, and better results than Yurman Baiyao（ P 〈 0.01 ）. Primary extracts of ethanol of O. japonicunwere short- ened obviously（P 〈0.01 ）. Petroleum extract and n-butanol extract of O. Japonicun couldn＇ t shortened the plasma recalcification time in vitro （ P 〉 0.05 ）, butcould significantly shortened coagulation time in vitro （ P 〈 0.01 ）. Water decoction of O. Japonicun didn＇ t demonstrated significantly effect on the plasma recalcification time as well as the coagulation time in vitro （ P 〉 0.05 ）. Conclusion： The acetic ether extract of O. japonicun has better hemostatic effect in vitro.