市售不同来源福林试剂对多肽含量测定结果的影响

Effects of Folin Reagent from Different Sources on the Content Determination of Polypeptide

目的:研究市售不同来源福林试剂对肌氨肽苷注射液中多肽含量测定结果的影响。方法:采用福林酚法测定多肽的含量;以牛血清白蛋白为对照制备标准溶液并绘制标准曲线;使用不同来源的福林试剂进行测定;应用统计学方法分析研究不同来源福林试剂对测定结果的影响。结果:多肽含量在0.024 9~0.224 1 mg·ml^(-1)浓度范围内线性关系良好,平均回收率为96.4%(RSD=1.5%,n=9);统计分析结果表明,使用不同来源福林试剂测得的结果有显著性差异。结论:市售不同品牌福林试剂对肌氨肽苷注射液中多肽含量测定的结果具有显著性影响,使用前应对福林试剂进行质量确认。

Objective： To study the effects of Folin reagent from different sources on the content determination of polypeptide in muscular amino acids and peptides and nucleosides injection. Methods： The Folin-reagent method was used to assay the content of polypeptide. Bovine serum albumin was used as the standard control to obtain the standard curve. Folin reagents from different sources were used and statistical methods were used to analyze the influence of Folin reagent from different sources on the experimental results. Results： There was a good linearity within the range of 0.024 9-0. 224 1 mg·ml^- 1, and the average recovery was 96.4% （RSD = 1.5%, n = 9）. Through the statistical analysis, the content of polypeptide was significantly different determined by Folin reagent from different sources. Conclusion ： Different brands of Folin reagent have significant effects on the experimental results of content determination of polypeptide in muscular amino acids and peptides and nucleosides injection. Therefore, quality verification should be done before the use of Folin reagent.