人乳头瘤病毒感染与原发性肺癌相关性研究

Association between human papillomavirus infection and lung cancer

目的 探索HPV感染与原发性肺癌的关联。方法 检测83对匹配的肺癌和癌旁正常组织中HPV感染情况。采用HPV高保守L1区PCR扩增和特异探针反向杂交相结合的方法，检测21种临床相关的HPV型别。应用配对 χ2检验分析肺癌与癌旁正常组织HPV检出率差异。应用 χ2检验及确切概率法分析不同性别、年龄、组织学类型、临床分期、吸烟和饮酒情况下肺癌组织HPV检出率差异。结果 7份肺癌组织和6份癌旁正常组织检出HPV，HPV阳性率在肺癌与癌旁正常组织间差异无统计学意义（P〉0.999）。在不同性别、年龄、吸烟、饮酒，不同病理类型、临床分期和淋巴结转移情况下，肺癌组织HPV检出率差异无统计学意义（P〉0.05）。结论 在福建人群中未发现HPV感染与原发性肺癌发生风险有明显关联。

Objective To investigate the association between human papillomavirus （HPV） and lung cancer. Methods We examined a series of 83 lung cancer patients with HPV DNA in both lung tumor specimens and adjacent normal specimens from Fujian province. Twenty-one of the most clinically relevant HPV types from the highly conserved L1 region of the viral genome were analyzed, using the PCR amplification and were followed by reverse hybridization with specific probes. Chi-square test of paired design was used to test the difference of HPV positive rates between lung cancer specimens and adjacent normal specimens. Chi-square test and Fisher＇s exact test were used to analyze the differences of HPV positive rate of tumor specimens on factors as gender, age, histological subtype, clinical stage, smoking status and alcohol consumption. Results HPV was detected in 7 of the 83 tumor specimens and in 6 of the paired normal lung tissues. There was no significant correlation between HPV and lung cancer （P〉0.999）. Neither demographic characteristics nor clinical features were found with significant differences on HPV in lung cancer tissues （P〉0.05）. Conclusion Our data showed that HPV was not significantly associated with the risk of lung cancer in Fujian province.