青蒿素抑制大鼠角膜移植排斥反应

Artemisinin Inhibiting Rejection of Rat Corneal Transplantation

目的探讨全身给予青蒿素,研究其对大鼠角膜移植排斥反应的抑制作用。方法本实验通过把Wistar大鼠和SD大鼠作为移植给予和接受的对象,建立角膜移植的动物模型。随机分为4组,正常组:不行角膜手术,也不药物干预;阳性对照组:给予1%羧甲基纤维素钠(CMC-Na)溶液1.5 m L;环孢霉素A组:给予环孢霉素A 10 mg/kg;青蒿素组:给予青蒿素600 mg/kg。术后在显微镜下观察各组角膜植片的状态,记录生存时间及排除反应指数,HE染色观察角膜植片的病理变化。结果阳性对照组、环孢霉素A组、青蒿素组角膜植片的平均存活时间分别为(11.67±0.67)d、(20.50±0.76)d、(18.67±0.67)d,环孢霉素A组、青蒿素组与阳性对照组比较,角膜植片的存活时间都明显增加。病理学检查结果:对照组可见角膜水肿,厚度增加,有较多的炎症细胞,周围可见新生血管,环孢霉素A组、青蒿素组的角膜植片均较阳性对照组明显好转,水肿程度减轻、有少量炎性细胞浸润,效果明显好于阳性对照组。结论全身给予青蒿素,可以使移植术后角膜植片存活的时间延长,使角膜的水减轻肿,减少炎细胞的浸润,从而能够抑制移植免疫排斥反应的发生。

Objective To investigate the systemic administration of artemisinin and study its inhibitory effect on corneal transplan-tation rejection of rats. Methods In this study, Wistar rats and SD rats were used as objects to receive transplantation, so as to estab-lish an animal model of corneal transplantation. They were randomly divided into four groups, normal group （ with neither corneal sur-gery nor drug intervention） , positive control group （ with 1. 5 mL of 1% sodium carboxymethylcellulose （ CMC-Na） solution） , cyclos-porine A group （ with 10 mg/kg cyclosporin A ） , and artemisinin group （ with 600 mg/kg artemisinin） . The state of corneal graft was observed under microscope after operation. Such indexes as the survival time and elimination reaction were recorded, and the pathologi-cal changes of corneal graft were observed by HE staining. Results The mean survival time of corneal graft of positive control group, cy-closporine A group and artemisinin group were 11. 67＋0. 67 days, 20. 50＋0. 76 days and 18. 67＋0. 67days respectively. Compared with positive control group, corneal graft survival time of cyclosporine A group and artemisinin group was significantly longer. Results of histopathologic examination showed that in the control group, corneal edema, increased thickness, more inflammatory cells and neo-vascularization were dectected. The corneal graft in the A group and artemisinin group was significantly better than that of the positive control group, with reduction of edema and a small amount of inflammatory cell infiltration, significantly better than those of the positive control group. Conclusion Systemic administration of artemisinin can obviously prolong corneal graft survival time, relieve edema, and reduce inflammatory cells infiltration, so as to inhibit transplantation immune rejection.