炎症性肠病模型大鼠结肠、血液中IL-17及脑内c-fos的表达规律研究

Expression of IL-17 in colon and blood, c-fos in cerebrum on rats with inflammatory bowel disease

目的研究炎症性肠病模型大鼠结肠黏膜、血液中IL-17水平的表达及脑内c-fos的变化,探讨IL-17在炎症性肠病中对脑-肠轴的影响。方法建立大鼠炎症性肠病模型,用荧光定量PCR方法检测不同发病时期大鼠结肠组织中IL-17 m RNA、脑组织中c-fos的表达水平,并采用ELISA方法检测IL-17蛋白在大鼠血清中的表达水平。结果炎症性肠病大鼠结肠中IL-17的m RNA水平、脑组织中的c-fos及血清中蛋白水平呈现一致变化,与结肠的大体形态损伤及病理变化呈正相关,即在病变最为严重的时候达到最大值,而随着炎症的消退逐渐下降。结论 IL-17参与了炎症性肠病的进程并且可能通过脑-肠轴互相作用来调控炎症性肠病。

Objective To observe the levels of IL-17 in colonic mucosa and blood, and brain c-fos changes of modeled rats with inflammatory bowel disease, and to investage the influence of IL-17 on the brain-gut axis of inflammatory bowel disease. Methods The experiment used artificially induced IBD （inflammatory bowel disease） rats as models. The mRNA levels of IL-17 in colon and the levels of c-fos in cerebrum were detected with the FQ-PCR （fluorescence quantitative PCR） methods, the expression level of IL-17 protein in serum was detected by ELISA. Results IL-17 mRNA level in colon tissue, the clos in cerebrum and the protein in serum had the similar temporal trends to the morphological injury and pathological changes of the colon. The above levels reached the maximum value when the lesion was the most serious, which declined with the inflammation subsidised. Conclusion The experiment shows that IL-17 maybe has a facilitating role in the process of IBD and interacts with brain-gut axis to regulate IBD.