白子草总黄酮及总多糖降血糖有效部位的提取工艺研究

Study on extraction technology of blood glucose-lowering components of total flavonoids and polysaccharides from Gynura divaricata(L.)DC.

目的：研究建立白子草总黄酮及总多糖降血糖有效部位的提取工艺。方法在单因素试验的基础上，考察提取次数、药材粒径、浸泡时间、料／液比、乙醇浓度、提取时间对白子草总黄酮提取率的影响，并通过L 9（3）^4正交试验优化总黄酮最佳提取工艺；以前期研究为基础，结合L9（3）^4正交试验，优化总多糖最佳提取工艺。结果白子草总黄酮最佳提取工艺为：药材最粗粉加20倍80％乙醇，浸泡0．5h，回流提取2次，每次1．0h ；总多糖最佳提取工艺为：醇提后药渣加20倍纯净水，80℃回流提取2次，每次1．0 h。该条件下白子草总黄酮及总多糖提取率均大于80％。结论本研究建立的白子草总黄酮及总多糖降血糖有效部位提取工艺稳定、重复性好，为白子草降血糖活性研究奠定了实验基础。

Objective To study and establish the extraction technology of hypoglycemic components of total flavonoids and polysaccharides from Gynura divaricata （L .） DC ..Methods Based on the singer factor test ,the influences of extracting times ,diameter of drug powder ,soaking time ,solid-liquid ratio ,ethanol concentration ,and extracting time on extraction ratio of total flavonoids were studied .The best extraction technology of total flavonoids from Gynura divaricata （L .） DC .was es-tablished through L9 （3）4 orthogonal tests .On the base of previous studies combine with L9 （3）4 orthogonal tests ,the best ex-traction technology of total polysaccharides from Gynura divaricata （L .） DC .was established .Results The optimized extrac-tion technology of total flavonoids was confirmed as follows ：drug powder of less than 10 mesh diameter were soaking 0 .5 hour with 80% ethanol ,and solid-liquid ratio 1∶20 ,and extracting twice ,each time one hour .The optimized extraction technology of total polysaccharides was confirmed as follows ：add 20 times water to the residue of ethanol ,extraction temperature 90℃ , and extracting twice ,each time one hour .The extraction rate of both total flavonoids and polysaccharides were more than 80% .Conclusion The extraction technology of hypoglycemic components of total flavonoids and polysaccharides from Gynura divaricata （L .） DC .established in this study is stable ,reproducible ,and lays a foundation for the further studies on hypogly-cemic components in Gynura divaricata （L .） DC .