新疆白刺叶中异鼠李素-3-芸香糖苷的含量分析

Quantitative analysis of isorhamnetin-3-rutinoside in leaves of Nitraria Spp. from Xinjiang region

目的：分析新疆产白刺叶中黄酮类成分异鼠李素唱3唱芸香糖苷的含量，对新疆白刺的品质评价和质量控制提供依据。方法采用 HPLC 法对新疆白刺叶中异鼠李素唱3唱芸香糖苷进行含量分析。色谱柱：Diamonsil C18柱（4．6 mm ×250 mm ，5μm）；流速：1 ml／min；柱温：26℃；检测波长：360 nm。结果新疆白刺叶中异鼠李素唱3唱芸香糖苷在0．007813～1 mg／ml的范围内线性关系良好，平均加样回收率为100．13％，RSD＝3．02％。结论异鼠李素唱3唱芸香糖苷在白刺叶中的含量存在较大的种内和种间差异。该方法操作简单，检测结果准确，重复性好，可作为新疆白刺叶中异鼠李素唱3唱芸香糖苷的定量分析方法。

Objective To provide supporting data for the quality control and evaluation of Nitraria Spp .from Xinjiang region by analyzing the content of isorhamnetin-3-rutinoside in its leaves .Methods HPLC method was used for the determina-tion of isorhamnetin-3-rutinoside in leaves of Nitraria Spp .Results There is a good linear relationship in the range of 0 .007813~1 mg/ml .The average recovery rate is 100 .13% （RSD=3 .02% ） .Conclusion There were large interspecific and intraspecific differences of isorhamnetin-3-rutinoside content in leaves of Nitraria Spp from Xinjiang region .A simple method was identified to give accurate and reproducible results .This method can be used for quantitative analysis of isorhamnetin-3-ru-tinoside content in leaves of N itraria Spp .