气相色谱-质谱联用法测定蓍草中的脂溶性成分

Analysis of liposoluble chemicals fromAchillea Alpina L. by GC-MS

目的采用气相色谱-质谱联用法(GC-MS)对蓍草脂溶性成分进行结构分析和鉴定。方法分两步提取蓍草,先用95%乙醇超声提取,再用石油醚萃取,得到其脂溶性成分后进行气相色谱检测。采用GC-MS法,通过NIST10谱库检索,同时结合人工解析,鉴定蓍草脂溶性成分的化学结构,并按峰面积归一化法计算各化合物在脂溶性成分中的相对百分含量。结果从蓍草中共分离鉴定出脂溶性成分29种,占石油醚萃取总量的82.74%。主要成分及其含量分别为表木栓醇(33.16%)、乙酸羽扇醇酯(10.99%)、棕榈酸(7.98%)、豆甾醇(3.68%)、硬脂酸(3.55%)、木栓酮(3.51%)等。结论该方法简便、准确,可用于蓍草中脂溶性成分的测定,并为进一步探讨蓍草药理作用及合理利用该植物资源提供了依据。

Objective To identify the chemical structures for the components in the liposoluble portion of Achillea alpi-na L .by gas chromatography/mass spectrometry （GC-MS） .Methods Achillea alpina L .was extracted with 95% alcohol and petroleum ether .The chemical compositions in the extract were analyzed with GC-MS .The structures were identified by comparing to the authentic samples and searching NIST 10 database .The relative content of each compound in the extract was calculated by area normalization method .Results Twenty-nine compounds were separated and identified ,which accounted for 82 .74% of petroleum ether extract .The major constituents were octamethylpicen-3-ol （33.16% ） ,Lup20（29）-en-3-ol ,acetate （3b） （10 .99% ） , n-Hexadecanoic acid （7 .98% ） , Stigmasterol （3 .68% ） , Octadecanoic acid （3 .55% ） , Friedelan-3-one （3 .51% ） .Conclusion This simple and accurate method can be used for the assay of liposoluble chemicals from Achillea alpi-na L .The results provide a foundation for pharmacological research and better use the resource of Achillea alpina L .