TG酶、Pepsin酶、Papain酶交联磁性微球对四环素吸附性研究

Study on adsorption of tetracycline by magnetic microsphere crosslinked with TG,Pepsin and Papain

利用戊二醛将谷氨酰胺转氨酶(TG)、胃蛋白酶(Pepsin)、木瓜蛋白酶(Papain)交联负载到氨基化磁性微球(AMM)上,制备出不同的固载酶的磁性吸附剂;使用扫描电子显微镜与红外光谱分析其结构特征,并研究酸度、初始浓度、吸附时间及温度对水中四环素吸附性的影响。结果表明:三种材料的静态吸附均为吸热过程,符合二级动力学方程及Freundlich吸附等温线,吸附机理为多层的化学吸附过程;在相同吸附温度下,吸附速率顺序为Pepsin-AMM>Papain-AMM>TG-AMM;当吸附温度为318 K时,吸附均为自发过程,其最大吸附量分别为99.72、64.97和67.52 mg/g。

Different enzyme-immobilized magnetic adsorbents were prepared by using glutaraldehyde to crosslink TG, Pepsin and Papain on amination magnetic microspheres. The adsorbents structure characteristics were analyzed by scanning electron microscope and infrared spectroscopy.The effect of initial concentration, adsorbent temperature,time and acidity on the adsorption of tetracycline in aqueous solution was investigated. Experimental results showed that the static adsorption accorded with the Pseudo-second-order kinetic aqd Freundlich adsorption isotherm, adsorption mechanism was not only multilayer chemical adsorption process but also endothermic process.Under the same adsorption temperature,the adsorption rate of order was Pepsin-AMM 〉 Papain-AMM 〉 TG-AMM. In the adsorption temperature was 318 K, adsorption process was spontaneous,the maximum adsorption capacities were 99.72,64.97 and 67.52 mg/g.