大孔树脂富集酸枣仁总黄酮工艺及其体外抗氧化性研究

Study on purification technology for total flavonoids from the seeds of Ziziphus jujuba Mill. with macroporous resins and in vitro antioxidant ability

研究大孔吸附树脂富集纯化酸枣仁总黄酮的最佳条件,并进行了总黄酮体外抗氧化能力的测定。利用静态吸附和动态吸附实验对5种不同极性的大孔吸附树脂进行筛选,并对上样液质量浓度、上样量、洗脱剂体积分数、洗脱剂体积以及洗脱剂流速等条件分别进行考察。采用DPPH自由基和ABTS+自由基的清除率以及铁氰化钾的还原能力作为指标考察纯化后总黄酮的体外抗氧化能力。结果表明AB-8大孔吸附树脂为纯化酸枣仁总黄酮的最佳树脂,纯化工艺为上样液质量浓度1.99 mg/m L,上样量50 m L,洗脱剂体积分数50%乙醇,洗脱剂体积50 m L,洗脱剂流速1 m L/min。抗氧化结果显示总黄酮对DPPH和ABTS+自由基具有明显的清除能力(IC50值为0.70 mg/m L和0.15 mg/m L),并对铁氰化钾表现出了较强的还原能力。

Study on optimum conditions of the purification of total flavonoids from the seeds of Ziziphus jujuba Mill. with macroporous adsorption resin.Meanwhile, to analyze the in vitro antioxidant capacities of the flavonoids.Five different polarity macroporous adsorption resin were selected by using static adsorption and dynamic adsorption tests, and the total flavonoids concentration of sample, sample volume, the volume fraction of the elution, the volume of elution and the flow rate were investigated, respectively.The flavonoids were evaluated by three models of antioxidant activity：DPPH. ,ABTS ＋ · and K3Fe（CN）6 with Vc as the positive control.The results indicated that AB-8 macroporous adsorption resin was selected as the purification of the total flavonoids of Ziziphus jujuba Mill.The optimum technology conditions were as follows： the total flavonoids concentration of sample was 1.99 mg/mL, sample volume was 50 mL,the volume fraction of the elution was 50% ethanol,the volume of elution was 50 mL, and the flow rate was 1 mL/min after purification.Compared with Vc,the fiavonoids from Ziziphus jujuba Mill.had stronger capacity of scavenging on DPPH. ,ABTS＋. （IC5o 0.70 mg/mL,0.15 mg/mL）and showed a high reduction capacity.