响应面法优化紫萁多糖提取工艺及体外抗癌活性研究

Optimization of extraction of polysaccharides from Osmunde japonica leaves by response surface methodology and their inhibitory effects on tumor

本文意在优化紫萁多糖的提取工艺,并探讨紫萁多糖对癌细胞的作用。以超声-微波协同萃取为方法,料液比、微波功率和提取时间为考察因素,在单因素实验的基础上,设计响应面Box-Benhnken中心组合实验,对紫萁多糖提取工艺参数进行优化,得到优化紫萁多糖的提取条件:料液比1∶35(g/m L),微波功率353 W,提取时间250 s时,紫萁多糖的提取率为0.236%。以体外细胞毒性实验方法研究紫萁多糖对Hep G-2癌细胞的抑制作用,当紫萁多糖浓度为0.001 mg/m L时,对Hep G-2癌细胞的抑制率为18.13%,在10 mg/m L时抑制率为72.65%,IC50值为0.474 mg/m L。

The study was to explore the optimum processes of extraction polysaccharides from Osmunda japonica leaves and the effect of Polysaccharides on cancer cells.Using the ultrasonic microwave synergistic extraction, solid-liquid ratio, microwave power and extraction time as factors, based on the single factor experiment, the response surface methodology （RSM） Box- Benhnken center combination experimental design was employed to optimize the extraction of polysaccharides of Osmunda japonica.The results of optimum extraction conditions were as follow：the solid-liquid ratio was 1：35 （g/mL）, microwave power 353 W, extraction time was 250 s.Under these conditions, the extraction yield was 0.236%. According to the Cytotoxicity test, the inhibitory effect of polysaccharides from Osmunda japonica on the HepG-2 cancer cells had been studied.The results of cytotoxicity test indicated that the concentration of polysaccharides were 0.001 mg/mL, 10 mg/mL separately, the inhibition rate was 18.13% ,72.65% respectively.The vaule of IC50 was 0.474 mg/mL.