金银花提取物对中波紫外线辐射致雄性小鼠生殖系统损伤的保护作用

Protective effects of honeysuckle extract against the UVB-induced radiation damage to reproductive system in male mice

研究金银花提取物对中波紫外线(UVB)辐射致雄性小鼠生殖系统损伤的保护作用。将60只雄性小鼠随机分为6组,分别为空白对照组、金银花提取物单独处理组、辐射模型组、金银花提取物低、中、高剂量处理组。其中金银花提取物单独处理组和低、中、高剂量组每天上午灌胃400、200、400和600 mg·kg-1·d-1的金银花提取物,空白和辐射模型组灌胃生理盐水,除空白和金银花提取物单独处理组外,其余4组均在下午接受3 h的UVB(285 nm)照射。实验处理30 d后,测定各组小鼠睾体比、精子活率、睾丸组织病理学变化及超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量。结果表明,与空白对照组相比,辐射模型组小鼠睾体比和精子活率均显著下降(p<0.05),睾丸曲细精管生精上皮排列疏松,可见生精细胞脱落至管腔,睾丸组织SOD活性显著降低而MDA含量显著升高(p<0.01)。与辐射模型组相比,金银花提取物低、中、高剂量处理组小鼠睾体比和精子活率均显著升高(p<0.05),生精上皮排列越来越致密,睾丸组织SOD活性显著增加而MDA含量显著下降(p<0.01),并且呈现良好的剂量-效应关系。以上结果表明,金银花提取物对UVB辐射致雄性小鼠生殖系统损伤有一定的保护作用。

The aim of this study was to investigate the protective effects of honeysuckle extract against the Ultraviolet B （UVB）- induced radiation damage to reproductive system in male mice. Sixty male mice were randomly divided into 6 groups, the blank control group, the honeysuckle extract single treatment group, the radiation model group and the honeysuckle extract low, middle, high dosage-treated groups. The mice in honeysuckle extract single treatment group and low, middle, high dosage-treated groups were given 400,200,400 and 600 mg· kg-1· d-1 honeysuckle extract by intragastric administration respectively. The blank control and radiation model group were given normal saline.The mice were all given orally in the morning.The rest of the four groups were given 3 hours of UVB （285 mm） radiation in the afternoon except the blank control and honeysuckle extract single treatment group.After 30 days,the testis to body ratio, sperm motility rates, histopathological changes in testis,the SOD activity and MDA content of testicular tissue were determined.When compared to the blank control group, the testis to body ratio and sperm motility rates of mice in the radiation model group declined significantly（p 〈 0.05）.The spermatogenic epitheliums were loosely-arranged and spermatogenic cells were fell into the tubules,the SOD activity degraded and MDA content raised significantly as well （p 〈 0.01 ）.The testis to body ratio, SOD activity increased, MDA content decreased significantly in the honeysuckle extract single treatment group（p〈0.05）.Compared to the radiation model group, with the increase of honeysuckle extract dosage, the testis to body ratio and the sperm motility rates in the honeysuckle extract-treated groups raised significantly（ p 〈 0.05） ,the spermatogenic epitheliums were more and more impact,the SOD activity raised and the MDA content of testicular tissue degraded notably（p 〈0.01）, and all these changes showed obvious dose-effect relationship.The results suggest that honeysuckle extract had protective effects against the UVB-induced radiation damage to reproductive system in male mice.