基因组磷硫酰化修饰的研究进展及展望

Research progress and prospects of phosphorothioate modification-A review

DNA磷硫酰化修饰是DNA骨架上的第一例生理修饰。该修饰由dnd ABCDE编码的5个蛋白协同作用,以硫原子取代DNA磷酸二酯键上一个非桥接的氧原子。研究发现,DNA磷硫酰化修饰广泛存在于各种微生物中,在不同细菌中存在序列特异性,且具有R_P空间构象专一性。近年来,对DNA磷硫酰化修饰的研究取得了一系列的成果。为了对DNA磷硫酰化修饰有一个系统全面的了解,本文将就这一特殊生理修饰的发现过程,研究进展,未来所面临的机遇及挑战作一个简要的概述。

DNA phosphorothioate modification was the first reported physiological modification in the DNA backbone. Five putative proteins encoded by the five-member dnd ABCDE gene cluster replaced the non-bridging oxygen in the sugar-phosphate backbone with a sulfur. Phosphorothioate modification occurrs in sequence-selective and Rp stereo-specific manner in diverse bacterial stains. In recent years, researchers have made systemic achievements in this area. To have a comprehensive understanding of this unusual modification, we reviewed the discovery and research progress in DNA phosphorothioate modification and also discussed opportunities and challenges in the future.