摘要
DNA磷硫酰化修饰是DNA骨架上的第一例生理修饰。该修饰由dnd ABCDE编码的5个蛋白协同作用,以硫原子取代DNA磷酸二酯键上一个非桥接的氧原子。研究发现,DNA磷硫酰化修饰广泛存在于各种微生物中,在不同细菌中存在序列特异性,且具有R_P空间构象专一性。近年来,对DNA磷硫酰化修饰的研究取得了一系列的成果。为了对DNA磷硫酰化修饰有一个系统全面的了解,本文将就这一特殊生理修饰的发现过程,研究进展,未来所面临的机遇及挑战作一个简要的概述。
DNA phosphorothioate modification was the first reported physiological modification in the DNA backbone. Five putative proteins encoded by the five-member dnd ABCDE gene cluster replaced the non-bridging oxygen in the sugar-phosphate backbone with a sulfur. Phosphorothioate modification occurrs in sequence-selective and Rp stereo-specific manner in diverse bacterial stains. In recent years, researchers have made systemic achievements in this area. To have a comprehensive understanding of this unusual modification, we reviewed the discovery and research progress in DNA phosphorothioate modification and also discussed opportunities and challenges in the future.
出处
《微生物学报》
CAS
CSCD
北大核心
2016年第12期1831-1839,共9页
Acta Microbiologica Sinica
基金
国家自然科学基金(31520103902
31170070
31222001
31170049)~~
关键词
磷硫酰化修饰
DNA骨架修饰
phosphorothioate modification
modification on DNA backbone