西安地区女性献血者HLA抗体筛查分析

A Screening Analysis of HLA Antibodies in Female Donor Population in Xi'an Area

目的对西安地区部分女性献血者的H L A抗体进行检测并分析,为提高输血的安全性提供实验数据。方法随机收集女性无偿献血者标本202份,采用ELISA技术对HLA-Ⅰ类、-Ⅱ类抗体进行检测;并对有无妊娠史的献血者HLA抗体产生的差异进行统计分析。结果 202例女性献血者中,37例HLA抗体阳性(18.3%)。有妊娠史女性献血者HLA抗体阳性率(30.9%)明显高于无妊娠史女性献血者(3.3%),且随着妊娠次数的增加,HLA抗体产生的机率升高。但是有无妊娠史与HLA抗体产生类型没有关联。结论女性无偿献血者HLA抗体阳性率较高,提示开展女性献血者HLA抗体筛查具有一定的临床意义。

Objective To detect and analyze antibodies to HLA in female donators in Xi＇an,and provide experimental data for improving the security of blood transfusion.Methods Serum specimens of202 women donators were randomly collected. and tested for HLA-Ⅰand HLA -Ⅱ antibodies using ELISA in order to reveal the differences of HLA antibodies between blood donors of non-pregnancy and those of pregnancy.Results For202 women of donations,HLA antibody of37 donators were positive（18.3%）. The HLA antibody positive rate in pregnant women was significantly higher（30.9%） than that in non- pregnancies,The prevalence of anti-HLA increased with the frequency of pregnancies Conclusion The female blood donators have a high positivity of anti-HLA,indicating a clinical implication of anti-HLA screening for safe transfusion in this target population.