雷公藤甲素防治大鼠青光眼术后滤过泡纤维化的临床观察

Clinical observation of triptolide in prevention and cure fibrosis of bleb in rats after glaucoma surgery

目的探讨雷公藤甲素防治大鼠青光眼术后滤过泡纤维化的可行性。方法选取Wistar大鼠100只分为两组,对照组和观察组各50例。采用房水释放联合激光房角光凝法建立青光眼大鼠模型,然后所有大鼠均进行青光眼手术。手术后,观察组大鼠使用雷公藤甲素预防治疗青光眼术后滤过泡纤维化,对照组大鼠使用5-氟尿嘧啶预防治疗青光眼术后滤过泡纤维化。观察比较防治滤过泡纤维化效果。结果观察组大鼠的眼压在手术后第1天与对照组相比无差异(P>0.05),在第6天、14天低于对照组(P<0.05),观察组大鼠滤过泡面积在手术后第1天、6天、14天均小于对照组大鼠(P<0.05);观察组大鼠的治疗后滤过泡分型Ⅰ型和Ⅱ型均优于对照组,Ⅲ型和Ⅳ型均低于对照组(P<0.05);观察组术后不良反应发生率为12.16%,低于对照组22.86%(P>0.05)。结论雷公藤甲素防治大鼠青光眼术后滤过泡纤维化效果明显,且安全性较高,值得临床广泛运用推广。

Objective To investigate the feasibility of triptolide in prevention and cure rats glaucoma surgery fibrosis. Methods 100 cases of Wistar rats were divided into two groups, with 50 cases in the control group and the observation group. Glaucoma rat model were built by aqueous release combined with laser photocoagulation, and all rats underwent glaucoma surgery. After surgery, the rats in the observation group were observed their triptolide preventive treatment in glaucoma surgery fibrosis, the control rats were observed their 5-fluorouracil preventive treatment in glaucoma surgery fibrosis. The effects of prevention and treatment of bleb fibrosis were compared. Results The intraocular pressure of rats in observation group in the first day after surgery compared with the control group has no significant difference （P 〉0. 05） , on the 6t h 14th day it was lower than the control group rats （P 〈0. 05）. Filtration area in the observation group on first, 6th day, 14th days after surgery, was less than the control rats （ P 〈 0. 05 ） ; In the observation group, the type Ⅰ and type Ⅱ of filtering bleb were better than those of the control group, the type Ⅲ and type Ⅳ were lower than those of the control group （ P 〈 0. 05） ; The adverse reaction rate was 12. 16% in observation group, it was lower than the control group 22. 86% （P 〈0. 05）. Conclusion Triptolide in prevention and cure of rats glaucoma surgery fibrosis is obvious, and high security. It is worthy of promotion.