改善激光共焦扫描显微镜像质的方法

Method for improving image quality of laser confocal scanning microscope

为解决传统光学显微镜样本上每一点的图像都受到邻近点衍射或散射光干扰的问题,研发了一套基于C#WinForm控制平台进行连续扫描方式的激光共焦扫描显微镜(LCSM)系统,并且成功地对生物细胞进行了扫描成像。针对共焦显微镜图像像质不高的问题,提出合理选取探测器针孔直径,并通过高斯低通滤波、盲解卷积的方法,确保实现高像质。实验结果表明,基于上述方法改进后的LCSM具有较高图像质量,该方法简单易行,便于实施。

In order to overcome the interference of diffracted or scattered light from the adjacent point,a laser confocal scanning microscope(LCSM)system was developed based on the C#WinForm control platform.The system was used to image biological cells.In view of the low quality of confocal microscopic image,reasonable selection of detector pinhole diameter,Gaussian low pass filtering,and blind deconvolution were put forward to realize high resolution.Experimental results show that the improved LCSM has higher resolution,and the proposed method is simple and easy to implement.