伴发抑郁对支气管哮喘大鼠P物质及皮质醇表达的影响

Influence of bronchial asthma with coexisted depression on levels of cortisol and substance P in rats

目的探讨伴发抑郁行为后支气管哮喘大鼠体内P物质、皮质醇含量的变化。方法 Wistar大鼠随机分为对照组(C组)、哮喘组(A组)、哮喘伴发抑郁组(A+D组),采用卵蛋白(ovalbumin,OVA)激发法建立哮喘模型,通过慢性轻度不可预见性应激(CUMS)诱发抑郁,评价动物哮喘及抑郁表现,检测大鼠血液及肺组织P物质、皮质醇的含量。结果 1伴发抑郁后,哮喘大鼠肺部病变进一步加重。2与C组比较,A组、A+D组大鼠血浆及肺组织P物质含量均显著升高(P<0.05),且A+D组水平高于A组(P<0.05)。3与C组比较,A组血液及肺组织皮质醇含量明显降低(P<0.05);而与A组比较,A+D组血液及肺组织皮质醇含量显著升高(P<0.05)。结论 OVA激发复合CUMS可制备支气管哮喘伴发抑郁大鼠模型,该模型P物质、皮质醇含量均较单纯哮喘发生明显变化。

Objective To detect the changes in levels of cortisol and substance P in rats of asthma with coexisted depression. Methods Wistar rats were randomly divided into the control group （group C）, asthma group （group A） and asthma with coexisted depression group （group A＋D）. The group A and group A＋D were sensitized with ovalbumin for 4 weeks. Then the group A＋D was exposed to a CUMS procedure for another 4 weeks. Asthmatic manifestations and depressive-like behaviors were investigated. Levels of substance P and cortisol were measured by radioimmunoassay. Results ①The asthmatic manifestations of group A＋D were worse than the group A. ②Rats in group A and group A＋D had much higher levels of substance P than the rats in group C （P 〈 0.05）; group A＋D had even higher substance P levels than group A （P 〈 0.05）. ③Levels of cortisol in group A significantly decreased if compared to the group C （P 〈 0.05）; however, levels of cortisol in group A＋D significantly increased if compared to the group A （P 〈 0.05）. Conclusion Ovalbumin excitation combined with CUMS can establish asthma with coexisted depression moldel in rats, which had higher levels of substance P and cortisol than the rats only with asthma.