参苓白术颗粒对溃疡性结肠炎模型小鼠的保护作用

Protective Effect of Shenling Baizhu Granule on Ulcerative Colitis Model

目的:研究参苓白术颗粒(SLBZ)对葡聚糖硫酸钠(DSS)诱导的小鼠结肠炎保护作用,并探讨其作用机制。方法:清洁级Balb/c小鼠随机分为正常组,3 d模型组,3 d参苓白术颗粒组,7 d模型组,7 d参苓白术颗粒组。3,7 d参苓白术颗粒组均连续8 d ig SLBZ(9 g·kg^(-1)·d^(-1)),正常组与3,7 d模型组ig等体积生理盐水。模型组和参苓白术颗粒组在ig的最后3d用4%DSS饮水造模,3 d模型组与3 d参苓白术颗粒组在造模第3天检测指标,正常组与7 d模型组,7 d参苓白术颗粒组在造模第7 d检测相关指标,观察体重变化、粪便评分、病理学评分、凋亡细胞、黏液表达量、紧密连接蛋白(occludin)蛋白水平等。结果:与正常组比较,3 d模型组凋亡细胞数量增多,occludin蛋白减少(P<0.05);7 d模型组体重下降,粪便评分增高,病理学评分增高,凋亡细胞数量增多,occludin蛋白减少(P<0.05);与模型组比较,预防性连续ig 8 d SLBZ,3 d参苓白术颗粒组小鼠凋亡细胞表达量少,occludin蛋白增多(P<0.05);7 d参苓白术颗粒组小鼠体重升高,腹泻和血便症状明显好转,病理学评分减少,黏液分泌量多,凋亡细胞减少occludin蛋白表达量增多(P<0.05)。结论:预防性服用SLBZ可以缓解DSS诱导的结肠炎发病,其药理机制可能与SLBZ保护结肠黏膜屏障密切相关。

Objective： To study the protective effect and mechanism of Shenling Baizhu granule（ SLBZ） on ulcerative colitis in mice induced by dextran sulfate sodium（ DSS）. Method： Clean-grade Balb/c mice were randomly divided into control group,3 d model group,3 d SLBZ group,7 d model group and 7 d SLBZ group. 3 d and 7 d SLBZ groups were given SLBZ（ 9 g·kg-1·d -1） by gavage for 8 days,while control group and two model groups were given the same volume of saline. In all groups except the control group,animal models were established with 4% DSS water in the last 3 days. 3 d model group and 3 d SLBZ group were observed and tested on 3 d after modeling,while control group,7 d model group and 7 d SLBZ group were observed and tested on 7 d after modeling. The changes in body weight,stool index（ diarrhea and bloody stool）,pathological score,apoptosis index,mucus expression and occludin protein were observed. Result： Compared with the normal group,the amount of apoptotic epithelial cells in colonic mucosa of 3 d model group increased significantly,the expression of occludin was significantly reduced（ P 〈 0. 05）. In 7 d model group,body weight decreased significantly,stool index increased significantly,the pathological score increased,the amount of apoptotic cells increased,and occludin protein expression was significantly reduced（ P 〈 0. 05）. Compared with the model group, after preventive gavage of SLBZ suspension for 8 days,the amount of apoptotic epithelial cells in 3 d SLBZ group decreased,occludin protein expression increased significantly（ P 〈 0. 05）,body weight of mice in 7 d SLBZ group increased,diarrhea and bloody stool symptoms improved obviously,pathological score decreased,the amount of mucus secretion increased,apoptotic cells decreased,and the expression of occludin increased（ P 〈 0. 05）.Conclusion： The preventive administration with SLBZ could relieve the symptoms of colitis induced by DSS,which may be closely related to protection of SLBZ on colonic mucosal barrier.