聚焦超声对SD外阴慢性单纯性苔藓模型大鼠外阴皮肤P物质及P物质受体表达的影响

Effects of focused ultrasound on expression of substance P and neurokinkin 1 receptor in genital skin of SD rats with vulvar lichen simplex chronicus

目的探讨聚焦超声对SD外阴慢性单纯性苔藓模型大鼠外阴皮肤中P物质(SP)和P物质受体(NK1-R)表达的影响。方法参考Sally法建造40只雌性SD大鼠外阴慢性单纯性苔藓模型,并随机分为治疗组(n=20)和对照组(n=20)。治疗组予以聚焦超声治疗,对照组采用与治疗组相同的方法治疗,但仪器无功率输出。聚焦超声治疗2周后,取大鼠外阴皮肤,HE染色观察组织学改变,甲苯胺蓝染色观察肥大细胞总数及脱颗粒率,免疫组织化学方法检测SP和NK1-R的表达,比较两组指标差异。结果聚焦超声治疗2周后,治疗组75.00%(15/20)大鼠外阴皮肤转为正常,对照组10.00%(2/20)大鼠外阴皮肤转为正常,差异有统计学意义(2χ=17.289,P<0.05)。治疗组肥大细胞总数及脱颗粒率及外阴皮肤中SP和NK1-R表达水平均明显低于对照组(P均<0.05)。结论聚焦超声可抑制外阴慢性单纯性苔藓大鼠皮肤中SP的释放及NK1-R的表达,并降低肥大细胞总数,减少肥大细胞脱颗粒,减轻局部炎症反应。

Objective To explore the effects of focused ultrasound on the expression of Substance P （SP） and Neurokinin-1 receptor （NK1-R） in the genital skin of SD rats with vulvar lichen simplex chronicus （LSC）. Methods Fourty female rat models of LSC were established by Sally method, and then the rats were randomly divided into treatment group （n=20） and control group （n=20）. The rats in treatment group were treated by focused ultrasound, and those in control group received sham treatment. After focused ultrasound intervention, the histological changes of vulva were observed by using HE staining. The total number and the degranulated number of mast cells were identified by staining slides with toluidine blue. Immunohistochemistry was used to test the expression of SP and NK1-R. Differences between the groups were compared. Results After 2 weeks of focused ultrasound treatment, the rate of normal in treatment group was 75.00% （15/20） and that in control group was 10.00% （2/20）, there was statistically significant between two groups （χ^2=17.289, P〈0.05）. The total number and the degranulated number of mast cells, the expression of SP and NK1-R in treatment group were significantly lower than those in control group （all P〈0.05）. Conclusion Focused ultrasound can treat LSC through inhibiting SP and NK1-R expression, decreasing the total number and the degranulated number of mast cells and reducing the inflammatory reaction of tissues.