HPLC法同时测定金英黄归汤中芦丁、槲皮素和木犀草素的含量

Simultaneous Determination of Rutin,Quercetin and Mignonette in Jinying Huanggui Decoction by HPLC

目的：采用高效液相色谱（HPLC）法建立一种同时测定金英黄归汤中芦丁、槲皮素和木犀草素的含量。方法：采用Hypersil ODS2 C18色谱柱（150 mm×4.6 mm,5μm）;以甲醇-0.4%磷酸（40∶60）为流动相;检测波长为360 nm;柱温为40℃和;流速为0.8 m L·min^-1。结果：芦丁、槲皮素和木犀草素分别在350.00μg·mL^-1-21.88μg·mL^-1（R^2=0.999 8）、245.00μg·mL^-1-15.31μg·mL^-1（R^2=0.999 9）和240.00μg·mL^-1-15.00μg·mL^-1（R^2=0.999 9）范围内线性关系良好。中间精密度试验RSD分别为1.05%、1.16%和0.50%;稳定性试验RSD分别为2.00、%1.79%和1.60%;重复性试验RSD分别为1.78%、1.23%和1.84%;平均回收率分别为芦丁99.08%（RSD=0.81%）,槲皮素100.5%（RSD=1.82%）,木犀草素99.92（RSD=1.19%）。结论：建立的该测定方法简便快捷,准确可靠,重复性好,可用于同时测定金英黄归汤中芦丁、槲皮素和木犀草素的含量测定。

Objective：To establish the simultaneous determination methods of rutin,quercetin and mignonette in Jinying Huanggui Decoction by HPLC.Method： Hypersil ODS2 C18column（150 mm × 4.6 m,5 μm） was used.The mobile phase was methanol-0.4% phosphoric acid solution（40 ∶ 60）.The detection wavelength was set at 360 nm.The column temperature was 40 ℃.The flow rate was 0.8 m L·min^-1.Results：The linear ranges of rutin,quercetin and mignonette were 350.00 μg·mL^-1- 21.88 μg·mL^-1（R^2= 0.999 8）,245.00 μg·mL^-1- 15.31 μg·mL^-1（R^2= 0.999 9）and 240.00 μg·mL^-1- 15.00 μg·mL^-1（R^2= 0.999 9） respectively.The RSD values of intermediate precision test were 1.05%,1.16% and 0.50% respectively.The RSD values of stability test were 2.00%,1.79% and 1.60% respectively.The repeatability test results were 1.78%,1.23% and 1.84% respectively.The average recoveries of rutin,quercetin and mignonette were 99.08%（RSD = 0.81%）,100.5%（RSD = 1.82%） and 99.92（RSD = 1.19%） respectively.Conclusion This method could be simple,accurate,repeatable and suitable for the simultaneous determinations of rutin,quercetin and mignonette in Jinying Huanggui Decoction.