武汉地区人群血清游离脂肪酸参考区间的探索

Investigation on reference interval of serum non-esterified fatty acid among population in Wuhan area

目的初步建立武汉地区人群血清游离脂肪酸（NEFA）酶法参考区间。方法选择该院健康体检者1 250例作为研究对象,采用奥林巴斯AU5400生化分析仪及Sekisui酶法检测试剂检测血清NEFA。回归分析NEFA的影响因素,正态分布法估计NEFA 95%参考区间,并验证参考区间。结果回归分析显示体质量指数（BMI）及年龄对NEFA有显著影响（P〈0.01）。NEFA在大于或等于60岁最高,18-〈45岁次之,45-〈60岁最低;NEFA在BMI〈18.5kg/m2组最高。估计武汉地区健康人群NEFA 95%参考区间为204.6-975.2μmol/L。临床验证者的测定范围为242.0-831.2μmol/L,符合上述参考区间。结论初步建立武汉地区健康人群NEFA参考区间。

Objective To preliminarily establish the reference interval of serum non-esterified fatty acids（NEFA）among population in Wuhan area by enzymatic assay.Methods NEFA level of serum samples from A total of 1 250 individuals undergoing healthy physical examination in our hospital were selected as the research subjects.The Olympus AU5400 biochemistry analyzer and SEKISUI reagents were adopted to detect serum NEFA level.The regression analysis was adopted to analyze the NEFA influencing factors.The NEFA 95% reference interval was estimated by using the normal distribution method and the reference interval was verified.Results The regression analysis showed that the body mass index（BMI）and age had significant effect on NEFA（P〈0.01）.NEFA was highest in the group aged≥60years old,followed by the group of 18-45years old,and lowest in the group of45-60 years old;NEFA was highest in the group of BMI18.5kg/m2.The 95%reference interval of NEFA for healthy population in Wuhan area was estimated as 204.6-975.2μmol/L.The detected NEFA level for clinical verifiers was 242.0-831.2μmol/L,which conformed to the above reference interval.Conclusion The serum NEFA reference interval among healthy population in Wuhan area is primarily established.