改良TT增菌液在分离肠道致病菌沙门菌和志贺菌的应用评价

Application evaluation of modified TT enrichment broth in separation of intestinal pathogenic Salmonella and Shigella bacteria

目的分析改良TT增菌液在分离肠道致病中沙门菌和志贺菌的效果评价。方法采用常规接种麦康凯平板及SS平板,同时另外加种改良TT增菌液（硫代硫酸钠碳酸钙增菌液）两种不同方法筛查沙门志贺菌,并参考该院历年菌株分离情况进行血清凝聚。采用SPSS18.0统计软件包处理数据,比较两种不同粪便培养方法对沙门菌和志贺菌的分离率之间差异。结果 2013-2015年纳入统计分析粪便培养标本790例,采用常规方法分离出沙门菌属30例,阳性率3.80%;志贺菌属5例,阳性率0.63%;而采用改良TT增菌培养方法分离出沙门菌属77例,阳性率9.75%,志贺菌属7例,阳性率0.89%;采用改良TT增菌液培养方法沙门菌检出率是常规培养方法2.57倍,志贺菌属是1.41倍,两种方法对沙门志贺菌属的分离差异均有统计学意义（P〈0.05）。结论改良TT增菌液能够显著地提高粪便培养沙门菌和志贺菌的阳性分离率,为临床医生正确诊治患者提供较大帮助。

Objective To analyze the effect evaluation of modified TT enrichment broth in the separation of intestinal pathogenic Salmonella and Shigella bacteria.Methods The routine inoculation of Macconkey agar and SS agar were adopted,meanwhile two different methods for adding the modified TT enrichment broth（sodium thiosulfate and calcium carbonate）were used to screen Salmonella and Shigella bacteria.Serum coagulation was performed by referring to the bacterial isolation situation over the years.The SPSS 18.0software was adopted to process the data.Then the difference in the separation rate of Salmonella and Shigella bacteria between the two different stool culture methods was compared.Results Totally 790 stool culture samples during 2013-2015 were included into the statistical analysis,30 cases of Salmonella bacteria were isolated by adopting the routine method,the positive rate was 3.80%;5cases were Shigella bacteria,the positive rate was 0.63%;but 77 cases of Salmonella bacteria were isolated by adopting the modified TT enrichment broth method,the positive rate was 9.75%,7cases were Shigella bacteria,the positive rate was0.89%;the detection rate of Salmonella bacteria by adopting the modified TT enrichment broth method was 2.57 times of the conventional culture method,which of Shigella bacteria was 1.41 times,showing that the difference of Salmonella and Shigella bacteria isolation between the two different methods had statistical significance（P〈0.05）.Conclusion The modified TT enrichment broth can significantly improve the positive isolation rate of Salmonella and Shigella stool culture,which provides larger help for clinical doctor′s correct diagnosis and treatment of patients.