EPS8与FOXM1在子宫腺肌病中的表达及意义

Expression and significance of EPS8 and FOXM1 in adenomyosis

目的检测EPS8与FOXM1在子宫腺肌病组织标本中的表达情况并评价其临床意义。方法选取子宫切除术后的子宫腺肌病组织样本30例为实验组，其中增生期18例，分泌期12例。实验组中异位病灶区子宫内膜组织设为异位组，非异位区子宫内膜组织设为在位组。收集同时期仅为子宫肌瘤的术后标本30例为对照组，其中增生期20例，分泌期10例。采用免疫组化和荧光染色方法检测EPS8在各组子宫内膜组织中的表达；荧光染色方法和Western blot定量检测FOXM1在异位组子宫内膜的表达变化。结果异位组和在位组子宫内膜EPS8的表达水平均显著高于对照组（P〈0．01）；并且异位组增生期子宫内膜EPS8的表达明显高于分泌期（P〈0．05）；异位组与在位组相比，子宫内膜EPS8表达差异无统计学意义（P〉0．05）。FOXM1在异位组的表达水平较对照组显著升高（P〈0．01）。子宫腺肌病患者病变组织中EPS8和FOXM1的表达水平呈明显相关性（r=0．5958，P〈0．05）。结论EPS8和FOXM1在子宫腺肌病患者病变组织中表达显著上调，提示它们表达异常可能是促进子宫腺肌病患者子宫内膜细胞迁移并在异位子宫内膜不断增殖的重要原因之一，联合检测EPS8和FOXM1表达水平可能为子宫腺肌病的诊断、治疗和判断疾病的进展状况提供新的思路。

Objective To detect the expression of epidermal growth factor receptor pathway substrate 8 （EPS8） and forkhead box M1 （FOXM1） in adenomyosis samples and to evaluate its clinical significance. Methods A total of 30 adenomyosis samples were collected after hysterectomy and set as an experimental group, including 18 cases at the proliferative phase and 12 cases at the secretory phase. In the experimental group, ectopic endometrial tissue was set as an ectopic group, while eutopic endometrial tissue was set as a eutopic group. At the same time, 30 uterine leiomyoma samples were collected as a control group, including 20 cases at the proliferative phase and 10 cases at the secretory phase. Immunohisto- chemistry and fluorescence staining were used to detect the expression of EPS8 in the endometrial tissues of each group. Meanwhile, the expression of FOXM1 in the ectopic endometrium was detected by fluorescence staining and Western blotting. Results The levels of EPS8 in both the ectopic and eutopic groups were significantly higher than those in the control group （ P 〈 0.01 ） and the level of EPS8 in proliferative phase was significantly higher than that in the secretory phase （ P 〈 0.05 ）. However, compared with the eutopic group, the amount of EPS8 in the ectopic group was increased slightly, indicating no statistical difference （P 〉 0.05 ）. The quantity of FOXM1 in the ectopic group was significantly higher than that in the control group （P 〈0.01 ）. Furthermore, a significant correlation was found between the levels of EPS8 and FOXM1 in patients with adenomyosis （P 〈 0.05）. Conclusions The levels of EPS8 and FOXM1 were significantly up -regulated in adenomyosis samples, which indicate that their abnormal expression may contribute to the migration of endometrial cells which can proliferate continuously in the ectopic endometrium. Combined detection of the levels of EPS8 and FOXM1 may provide new ideas for diagnosis and treatment of adenomyosis and judgment the progression of the disease.