昆明市某医院2013～2014年住院婴幼儿感染星状病毒的分子流行病学研究

Molecular Epidemiology of Astrovirus Infection Among Hospitalized Infants in Kunming City,China,2013～2014

探讨昆明市某医院2013-2014年住院婴幼儿星状病毒（human astrovirus,HAstV）腹泻的感染情况,分析其分子流行病学特征。通过逆转录RT-PCR法,对63份婴幼儿腹泻样品和42份非腹泻样品进行星状病毒分型鉴定;并扩增1株HAstV全基因组序列。结果在63份样品中,HAstV检出率为41.27%（26/63）,以HAstV1型为主要流行株;42份对照组中,HAstV感染率为2.38%（1/42）;扩增获得1株HAstV全基因组序列,进化树分析为HAstV1型。本研究获得了昆明地区婴幼儿星状病毒腹泻流行病学数据,为昆明地区星状病毒腹泻的感染、控制提供了重要的理论依据。

We investigated the infection and molecular-epidemiologic characteristics of human astrovirus（HAstV）of hospitalized infants in Kunming City from the year 2013 to 2014.Infection and genotype of HAstV of 63 samples of diarrheal feces and 42 controls were analyzed by reverse transcription-polymerase chain reaction（RT-PCR）.The complete genome sequence of a HAstV strain was amplified and sequenced.The positive rate of HAstV in 63 feces samples was 41.27%（26/63）.The main circulating genotype of HAstV was HAstV1.Only 1sample was positive for HAstV in 42controls（2.38%）.A complete genome sequence of the HAstV strain was identified as HAstV1 by phylogenetic analyses.These data provide an important theoretical basis for the control of viral diarrhea in infants in Kunming City.