子宫内膜癌中DJ-1表达及其与预后的关系

Expression of D J-1 protein in endometrial carcinoma tissue and its correlation with prognosis

目的探讨人类帕金森基因-7（DJ-1）在子宫内膜癌中的表达及其与临床病理特征及预后的关系。方法收集湖南省肿瘤医院69例子宫内膜癌患者的临床病理资料，采用免疫组织化学方法检测DJ-1在69例子宫内膜癌及35例正常子宫内膜组织中的表达，并进行生存分析。结果（1）在子宫内膜癌组织中DJ-1的阳性表达率明显高于正常内膜组织（P〈0．05）；（2）DJ-1蛋白的高表达在子宫内膜癌中均与子宫内膜癌组织学分级、临床分期、肌层浸润深度、淋巴结转移等有关（P〈0．05）；（3）DJ-1低表达组5年生存率（S0．8％）明显高于DJ-1高表达组（51．2％，P〈0．05）；COX多因素分析显示DJ-1的表达强度不是子宫内膜癌患者生存时间的独立预后因素（P〉0．05）。结论DJ-1在子宫内膜癌组织中高表达，并与组织学分级、临床分期、肌层浸润深度、淋巴结转移等密切相关。DJ-1尚不足以成为子宫内膜癌的预后分子标志。

Objective To investigate the expression and clinical significance of DJ-1 protein in the endometrial carcinoma and delineate its functional roles in endometrial carcinoma progression. Methods Expression of DJ-1 was examined with immunohistochemical staining of 69 paraffin-embedded endometrial carcinoma samples who underwent surgery at Hunan province Tumor Hospital. Kaplan-Meier analysis was performed to describe the survival curve. Univariate and multivariate analyses were performed with COX proportional hazard model. Results （1） The expression of DJ-1 protein in endometrial carcinoma was significantly higher than that in normal endometrial tissues （ P 〈0. 05 ）. （2） There was positive correlation between the high expression of DJ-1 protein in endometrial carcinoma samples and the pathological grade, the International Federation of Gynecology and Obstetrics （FIGO） stage, depth of myometrial invasion, or lymph node metastasis （ P 〈 0.05 ）. （3） Kaplan-Meier analysis indicated that the cumulative 5-year survival rate in the low-DJ-1 expression group was 80. 8%, whereas it was only 51.2% in the high-DJ-1 expression group （P 〈0. 05）. COX analysis indicated that the expression of D J-1 was not the endometrial carcinoma independent prognostic factors for overall survival time （P 〉0. 05）. Conclusions The high expression of DJ-1 protein in endometrial carcinoma samples demonstrated a significantly positive correlation with the pathological grade, FIGO stage, depth of myometrial invasion, and lymph node metastasis. However, DJ-1 is not enough to be independent prognostic factor for overall survival time.