切花玫瑰红唇的组织培养快繁技术研究

Study on Technology of Rapid Propagation in Tissue Culture of Cut Rose Hongchun

以切花玫瑰红唇的带腋芽茎段为材料,研究HgCl_2灭菌时间、茎段粗细度、基础培养基、6-苄氨基腺嘌呤(6-BA)、α-萘乙酸(NAA)、赤霉素(GA_3)、吲哚丁酸(IBA)、吲哚乙酸(IAA)、活性炭(AC)对切花玫瑰组织培养的影响,分析筛选出了一套能够育出具有优良综合素质切花玫瑰种苗的组织培养技术,建立了切花玫瑰的组织培养快繁技术体系。结果表明:不同因素对切花玫瑰组织培养的影响较大,尤其是对玫瑰的分化、增殖、生根和生长等植物形态发育的影响;外植体的最佳灭菌方法是用0.20%HgCl_2灭菌8min;初代的适合培养基为MS+6-BA 1.0mg/L+NAA 0.2mg/L+GA_3 2.0mg/L;增殖继代的最佳培养基为MS+6-BA 2.0mg/L+NAA 0.01mg/L+GA_3 1.0mg/L;生根的最佳培养基为1/2 MS+6-BA 0.5mg/L+IBA 1.0mg/L+AC 0.1%。该技术可推广应用于切花玫瑰工厂化组织培养育苗。

With stem segments of axillary buds in cut rose Hongchun as materials, experiments were carried out to study effects of 9 factors （HgCl2 sterilization time, basal medium, 6-BA, etc.） on cut rose tissue culture, and then to screen out a cut rose tissue culture technology, which could produce better quality seedlings, and eventually to establish a tissue culture and rapid propagation system in cut rose. The results showed that 9 factors had great effects on cut rose tissue culture; the best sterilization approach for explants was to treat 8 min by 0.20% HgCl2; suitable primary culture medium was MS＋6-BA 1.0mg/L＋NAA 0.2mg/L＋GA3 2.0mg/L; the best proliferation subculture medium was MS＋6- BA 2.0mg/L＋NAA 0.01mg/L＋GA3 1.0mg/L; and the best rooting medium was 1/2 MS＋6-BA 0.5mg/L＋IBA 1.0mg/ L＋AC 0.1%. These technologies are able to apply in the factory production of cut rose by plant tissue culture.