RTN4基因rs2864052和rs6545468与广西壮族人群鼻咽癌易感性相关研究

Association of RTN4 gene rs2864052 and rs6545468 with the susceptibility of nasopharyngeal carcinoma in Guangxi Zhuang population

目的:研究RTN4多态性rs2864052和rs6545468位点及其单倍型与广西壮族人群鼻咽癌易感性相关关系。方法:运用单碱基延伸PCR技术和DNA测序法对广西壮族282例鼻咽癌患者(鼻咽癌组)和199例健康体检者(对照组)的RTN4基因rs2864052和rs6545468位点进行基因分型,并分析RTN4基因的单倍型频率。结果:RTN4基因rs2864052存在AA、GA、GG三种基因型和A、G等位基因,其在鼻咽癌组与对照组中的基因型、等位基因频率的分布差异无统计学意义;rs6545468位点存在CC、GC、GG三种基因型和C、G等位基因,其在鼻咽癌组与对照组中的基因型、等位基因频率的分布差异亦无统计学意义。但单倍型分析发现鼻咽癌组单倍型AG频率显著低于对照组(P=0.004,OR=0.14,95%CI为0.31-0.68)。结论:RTN4基因多态性rs2864052和rs6545468位点的单倍型AG可降低广西壮族人群个体鼻咽癌易感性。

Objective：To study the relationship of the polymorphism of RTN4 gene rs2864052 and rs6545468 and haplotype with the susceptibility of nasopharyngeal carcinoma in Guangxi Zhuang population. Method：The pol- ymorphism of Nogo gene （rs2864052, rs6545468） and haplotype were analyzed using the method of single-base ex- tension PCR and DNA sequencing in 282 cases of nasopharyngeal carcinoma （NPC） and 199 healthy persons （con- trol group） in Guangxi Zhuang Autonomous Region. Result： There were no differences between the NPC＇S patients and controls in the genotype and allele frequencies of RTN4 gene rs2864052 site,or rs6545468 site. The frequency of AG haplotype in the NPC＇S patients was significantly lower than in the controls（P=0. 004, OR= 0. 14,95% CI=0. 31-0.68）. Conclusion： The haplotype AG of RTN4 gene rs2864052 and rs6545468 sites may reduce the risk of nasopharyngeal carcinoma in Guangxi Zhuang population.