植物Tnt1反转录转座子突变体库的特征及应用

The Application of Retrotransposon Tnt1 in Establishing a Plant Mutant Population

植物反转录转座子是转座子中的一大类。目前,在构建突变体库中应用较多的反转录转座子包括Tnt1、Tos17、Tto1和LORE1。该文比较了利用不同反转录转座子构建的植物突变体库的特征,并对目前应用较为广泛的Tnt1突变体库进行了详细的分析和总结,重点对豆科模式植物蒺藜苜蓿(Medicago truncatula)Tnt1突变体库的构建及其在固氮、代谢和发育等方面的应用进行了系统概括。该综述将有助于全面了解蒺藜苜蓿Tnt1突变体库的特征,并为豆科植物功能基因组学研究提供有益的参考。

Plant retrotransposons are among the largest transposon families. Tntl, Tos17, Ttol and LORE1 have been widely used in establishing mutant populations. In the present study, we summarized and compared various approaches for establishing mutant populations, especially Tntl retrotransposons. We focused on advances in establishing and using the Medicago truncatula Tntl mutant population, including nitrogen fixation, metabolism and development. Our study will benefit the comprehensive understanding and use of the M. truncatula Tntl mutant population and provide valuable references for researchers studying legume functional genomes.