红景天多糖对藏猪精液4℃保存效果的研究

Effect of Rhodiola Polysaccharides on Tibetan Boar Semen Preserved at 4℃ and Antioxidative

试验旨在研究红景天多糖对4℃藏猪精液品质和抗氧化保护作用影响,采用电刺激法采集健康藏猪精液,在改进的猪精液低温稀释液为基础液中,每1 000 m L添加红景天多糖0、3.0、5.0、7.0、9.0、11.0 g,将藏猪种公猪新鲜精液做4倍稀释后于4℃环境保存至第5天,以藏猪精子活力、精子畸形率、精子顶体完整率和质膜完整率为评价精液品质指标,以超氧化物歧化酶(Superoxidas dismutase,SOD)活力和丙二醛(Maleic dialdehyde,MDA)含量为评价抗氧化保护作用指标,探讨红景天多糖对4℃藏猪精液品质影响。结果显示:藏猪精液4℃保存到第5天时,9.0 mg/L红景天多糖添加组的精子活力、精子畸形率、精子顶体完整率和质膜完整率这4个指标均极显著好于其他组(P<0.01)。7.0 mg/L和11.0 mg/L添加组除精子活力差异显著外,其他指标差异不显著(P>0.05)。同时发现,9.0 mg/L红景天多糖添加组精子超氧化物歧化酶活力最高,5.0 mg/L、7.0 mg/L和11.0 mg/L组间无差异;精子MDA含量以9.0 mg/L红景天多糖添加组为最高,较7.0 mg/L和11.0 mg/L、5.0 mg/L、3.0 mg/L和对照组差异极显著(P<0.01),7.0 mg/L和11.0 mg/L添加组无差异。总之,在改进的猪精液稀释液中添加9.0 mg/L的红景天多糖可明显改善4℃环境保存藏猪精液品质和抗氧化保护作用,该试验研究为今后藏猪精液低温保存的生产应用奠定了基础。

This study was conducted to investigate the effects of Rhodiola Polysaccharides on Tibetan boar se-men preserved at 4 ℃ and antioxidative. The semen was collected from Tibetan boar by electrical stimulation.Add Rhodiola Polysaccharides 0 g, 3.0 g, 5.0 g, 7.0 g, 9.0 g, 11.0 g per 1 000 m L, in the improvement of pig se-men low temperature dilution liquid. Save to fifth days at 4 ℃ after dilution of fresh semen. Body integrity and membrane integrity rate of the top Tibetan pig sperm activity, sperm abnormal rate and semen quality evaluation index, to investigate the effect of Rhodiola Polysaccharides on the quality of semen quality at 4 ℃, the activity of Superoxidas dismutase（SOD）and Maleic dialdehyde（MDA）were the indexes to evaluate the protective effect of antioxidation. The results show that, to the fifth days,Tibetan boar semen preserved at 4 ℃, sperm motility, sperm abnormality rate, sperm integrity rate and plasma membrane integrity rate with add group of Polysaccharide 9.0mg/L from root of the root, these 4 indicators were significantly better than other groups（P〈 0.01）. The sperm motility was significantly（P〈 0.05） different between the 7.0 mg/L and 11.0 mg/L text groups, other indicators were not significant. Also, at the same time, we found that, addition of Polysaccharides from semen of low temperature dilution liquid of Tibetan boar semen, 9.0 mg/L Rhodiola Polysaccharides supplementation group sperm superoxide dismutase（SOD） activity is the highest, but there was no difference between 5 mg/L, 7 mg/L and 11 mg/L groups. 9.0 mg/L Rhodiola Polysaccharides supplementation group sperm MDA activity is the highest, compared with 7 mg/L and 11 mg/L, 5 mg/L, 3 mg/L and control group, the difference was very significant（P〈 0.01）, 7 mg/L and 11 mg/L add group no difference. In short adding 9.0 mg/L Rhodiola Polysaccharides to the improved pig semen diluent can significantly improve the semen quality and antioxidant protection at 4 ℃. The experimental study laid a foundation for the production and application of cryopreservation of boar semen in the future.