维生素E对4℃保存藏猪精液品质和抗氧化保护作用的影响

Effect of Vitamin E on Tibetan Boar Semen Preserved at 4℃ and Antioxidative

试验旨在研究维生素E对4℃藏猪精液品质和抗氧化保护作用影响,采用电刺激法采集健康藏猪精液,在改进的猪精液低温稀释液为基础液中,每1 000 m L添加维生素E 0、3.0、4.0、5.0、6.0 g,将藏猪种公猪新鲜精液做4倍稀释后于4℃环境保存至第5天,以藏猪精子活力、精子畸形率、精子顶体完整率和质膜完整率为评价精液品质指标,以超氧化物歧化酶(Superoxidas dismutase,SOD)活力和丙二醛(Maleic dialdehyde,MDA)含量为评价抗氧化保护作用指标,探讨维生素E对4℃藏猪精液品质影响。结果显示:维生素E 5.0 g/L添加组藏猪精子活力、精子畸形率、精子顶体完整率和质膜完整率均极显著好于其他试验组(P<0.01)。精浆超氧化物歧化酶活力和丙二醛含量以5.0 g/L维生素E添加组为最高;同时发现,5.0 g/L维生素E添加组精子超氧化物歧化酶活力与其他各组间差异不显著。总之,在改进的猪精液稀释液中添加5.0 g/L的维生素E可明显改善4℃环境保存藏猪精液品质和抗氧化保护作用,此试验研究为今后藏猪精液低温保存的生产应用奠定了基础。

This study was conducted to investigate the effects of vitamin E on Tibetan boar semen preserved at4 ℃ and antioxidative. The semen was collected from Tibetan boar by electrical stimulation. Add vitamin E 0 g,3.0 g, 4.0 g, 5.0 g, 6.0 g per 1 000 m L, in the improvement of pig semen low temperature dilution liquid. Save to fifth days at 4 ℃ after dilution of fresh semen. Body integrity and membrane integrity rate of the top Tibetan pig sperm activity, sperm abnormal rate and semen quality evaluation index, to investigate the effect of vitamin E on the quality of semen quality at 4 ℃, the activity of Superoxidas dismutase（SOD） and Maleic dialdehyde（MDA）were the indexes to evaluate the protective effect of antioxidation. The results show that, The sperm motility,sperm deformity rate, sperm motility rate and plasma membrane integrity rate of the vitamin E 5 g/L addition group were significantly better than other test groups（P〈0.01）, seminal plasma superoxide dismutase activity and MDA content was the highest in the 5 g/L vitamin E group. Also, at the same time, we found that, it was not significant difference between the other groups for sperm superoxide dismutase activity with 5 g/L vitamin E supplementation group. In short adding 5 g/L vitamin E to the improved pig semen diluent can significantly improve the semen quality and antioxidant protection at 4 ℃. The experimental study laid a foundation for the production and application of cryopreservation of boar semen in the future.